Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters.
Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters.
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DOI：
10.1186/1475-2859-9-18
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发表时间：
2010-03-19
影响因子：
6.4
通讯作者：
Trujillo-Roldán MA
中科院分区：
文献类型：
--
作者：
Valdez-Cruz NA;Caspeta L;Pérez NO;Ramírez OT;Trujillo-Roldán MA
基于p1和/或pr噬菌体lambda启动子的温度诱导表达系统由不耐热的cI857阻遏因子调控，已被广泛用于在原核生物细胞中生产重组蛋白。在这个表达系统中，外源蛋白的诱导是通过提高培养温度来实现的，温度一般在37℃以上。在外源蛋白过度表达的同时，温度的升高也会引起各种复杂的胁迫反应。许多研究报道了这种温度诱导表达系统的使用，但很少有人讨论重组蛋白生产和温度上移所引起的同时胁迫效应。了解这些反应的整体影响，对于制定改进的高产蛋白质生产和回收战略应该是有用的。本文综述了基于Pl和/或Prλ噬菌体启动子的热诱导表达系统的研究现状，重点介绍了表达载体、热诱导后在分子和生理水平上的应激反应以及影响蛋白质过表达的生物处理因素，包括培养操作变量和诱导策略。
The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic cells. In this expression system, induction of heterologous protein is achieved by increasing the culture temperature, generally above 37°C. Concomitant to the overexpression of heterologous protein, the increase in temperature also causes a variety of complex stress responses. Many studies have reported the use of such temperature inducible expression system, however only few discuss the simultaneous stress effects caused by recombinant protein production and the up-shift in temperature. Understanding the integral effect of such responses should be useful to develop improved strategies for high yield protein production and recovery. Here, we describe the current status of the heat inducible expression system based on the pL and/or pR λ phage promoters, focusing on recent developments on expression vehicles, the stress responses at the molecular and physiological level that occur after heat induction, and bioprocessing factors that affect protein overexpression, including culture operation variables and induction strategies.
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