Murine folliculogenesis in vitro is stage-specifically regulated by insulin via the Akt signaling pathway
Murine folliculogenesis in vitro is stage-specifically regulated by insulin via the Akt signaling pathway
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小鼠体外卵泡发生由胰岛素通过 Akt 信号通路进行阶段特异性调节
DOI：
10.1007/s00418-010-0708-8
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发表时间：
2010-05
影响因子：
2.3
通讯作者：
中科院分区：
文献类型：
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作者：
卵泡发生是哺乳动物发育过程中的一个重要过程，但对原始卵泡形成、生长和激活的机制知之甚少。本研究的目的是评估持续胰岛素暴露对16.5 dpc和3 dpp小鼠卵巢体外卵泡发生的影响。结果表明，5 μg/ml胰岛素可通过InsR/PI 3 K/Akt信号通路促进16.5dpc胎鼠卵巢Akt的磷酸化，促进原始卵泡的组装和激活。然而，5 μg/ml胰岛素的存在抑制Akt的磷酸化以及体外培养的3 dpp小鼠卵巢中卵泡的组装和激活过程。此外，体外培养的3dpp小鼠卵巢中加入胰岛素可抑制Pten的表达.总之，卵泡发育是阶段特异性调节胰岛素通过Akt信号通路在体外。
Folliculogenesis is a critical process during mammalian development, but little is known about the mechanisms underlying primordial follicular formation, growth, and activation. The objective of this study was to assess the effect of continuous insulin exposure on folliculogenesis in 16.5 dpc and 3 dpp mouse ovaries in vitro. The results demonstrated that 5 μg/ml insulin promotes the phosphorylation of Akt and the primordial follicular assembly and activation process via the InsR/PI3K/Akt signaling pathway in the 16.5 dpc fetal mouse ovaries cultured in vitro. However, the presence of 5 μg/ml insulin represses the phosphorylation of Akt and the follicular assembly and activation process in the 3 dpp mouse ovaries cultured in vitro. Furthermore, in vitro cultures of 3 dpp mouse ovaries with insulin can repress the expression ofPten. In conclusion, folliculogenesis was stage-specifically regulated by insulin via the Akt signaling pathway in vitro.
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影响因子：
64.5
作者：
Brunet, A;Bonni, A;Greenberg, ME
通讯作者：
Greenberg, ME
影响因子：
4
作者：
L. Sun;Z. Sun;P. Zhang;X. Zhai;J. Tang;Q. Pan;Q. Shi;W. Shen
通讯作者：
L. Sun;Z. Sun;P. Zhang;X. Zhai;J. Tang;Q. Pan;Q. Shi;W. Shen
影响因子：
56.9
作者：
Reddy, Pradeep;Liu, Lian;Liu, Kui
通讯作者：
Liu, Kui
影响因子：
4.8
作者：
Qi Zhang;Xiangyuan Wang;D. Wolgemuth
通讯作者：
Qi Zhang;Xiangyuan Wang;D. Wolgemuth
DOI：
10.1016/s0021-9258(17)37680-9
发表时间：
1994-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Vlahos;W. Matter;K. Hui;R. F. Brown
通讯作者：
C. Vlahos;W. Matter;K. Hui;R. F. Brown