Regulation of pyrimidine salvage in Aspergillus nidulans: A role for the major regulatory gene areA mediating nitrogen metabolite repression
Regulation of pyrimidine salvage in Aspergillus nidulans: A role for the major regulatory gene areA mediating nitrogen metabolite repression
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构巢曲霉中嘧啶补救的调节：主要调节基因 areA 介导氮代谢物抑制的作用
DOI：
10.1007/bf00328713
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Arst
中科院分区：
文献类型：
--
作者：
P. M. Shaffer;H. Arst
胸腺嘧啶7-羟化酶是一种α-酮戊二酸依赖性双加氧酶，其合成既受氮代谢抑制又受氧抑制，而另一种嘧啶补救途径双加氧酶嘧啶脱氧核苷2′-羟化酶的合成既不受氮代谢抑制也不受氧抑制。areA 300是构巢曲霉中调节氮代谢物阻遏的正作用调节基因areA的等位基因，显著提高了胸腺嘧啶7-羟化酶的水平，可能至少部分地减轻了氮代谢物阻遏和氧阻遏。areA 300对嘧啶脱氧核糖核苷2′-羟化酶的水平几乎没有影响，但确实使胸腺嘧啶、胸苷和脱氧尿苷的净摄取能力提高了两倍。areA 300被选择为允许胸腺嘧啶补充嘧啶营养缺陷型，并且发现其也允许通过胸腺嘧啶、其他嘧啶核苷和嘧啶补救中间体进行补充。这是第一个报道的证据，面积控制活动（-ies）不直接涉及氮源的利用。
SummaryThe synthesis of thymine 7-hydroxylase, an α-ketoglutarate dependent dioxygenase, is subject both to nitrogen metabolite repression and to oxygen repression, while synthesis of the other pyrimidine salvage pathway dioxygenase, pyrimidine deoxyribonucleoside 2′-hydroxylase, is subject to neither. areA300, an allele of the positive acting regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans, considerably elevates levels of thymine 7-hydroxylase, probably alleviating at least partly both nitrogen metabolite repression and oxygen repression. areA300 has little or no effect on levels of pyrimidine deoxyribonucleoside 2′-hydroxylase but does elevate net uptake capacities for thymine, thymidine and deoxyuridine two-fold. areA300 was selected as allowing thymine to supplement a pyrimidine auxotrophy and was found to allow supplementation by thymidine, other pyrimidine nucleosides and pyrimidine salvage intermediates as well. This is the first reported evidence for areA control over an activity(-ies) not directly concerned with nitrogen source utilization.
真菌中氮代谢和基因表达的调节。
DOI：
10.1128/mr.45.3.437-461.1981
发表时间：
1981
期刊：
Microbiological reviews
影响因子：
--
作者：
Marzluf,GA
通讯作者：
Marzluf,GA
鉴定粗糙脉孢菌氮控制回路主要调节基因的产物作为核 DNA 结合蛋白。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Grove,G;Marzluf,GA
通讯作者：
Marzluf,GA