Reduced pathogenicity of the SARS-CoV-2 omicron variant in hamsters.
Reduced pathogenicity of the SARS-CoV-2 omicron variant in hamsters.
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DOI：
10.1016/j.medj.2022.03.004
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发表时间：
2022-04-08
期刊：
影响因子：
--
通讯作者：
Barouch DH
中科院分区：
文献类型：
--
作者：
McMahan K;Giffin V;Tostanoski LH;Chung B;Siamatu M;Suthar MS;Halfmann P;Kawaoka Y;Piedra-Mora C;Jain N;Ducat S;Kar S;Andersen H;Lewis MG;Martinot AJ;Barouch DH
严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）Omicron（B.1.1.529）变体已被证明具有高度传播性，并在世界许多地区超过了Delta变体。早期的报告也表明，Omicron可能导致人类临床疾病的严重程度较低。在这里，我们表明，Omicron比以前的SARS-CoV-2变种在叙利亚金仓鼠的致病性低。仓鼠接种SARS-CoV-2 Omicron或其他SARS-CoV-2变体。跟踪动物的体重减轻，并评估上呼吸道和下呼吸道组织的病毒载量和组织病理学。用SARS-CoV-2 WA 1/2020、α、β或δ毒株感染仓鼠，导致第4天体重减轻4%-10%，第6天体重减轻10%-17%。相比之下，用两种不同的Omicron攻毒储备液感染仓鼠，即使在高攻毒剂量下，也未导致任何可检测到的体重减轻。与WA 1/2020感染相比，Omicron感染导致上呼吸道和下呼吸道大量病毒复制，但肺实质中的病毒载量较低，肺部病理学减轻。这些数据表明，与先前的SARS-CoV-2变体相比，SARS-CoV-2 Omicron变体可能导致强烈的上呼吸道感染，但不太严重的下呼吸道临床疾病。这项研究的资金由NIH资助CA 260476，马萨诸塞州病原体准备联盟，Ragon研究所和Musk基金会提供。SARS-CoV-2 Omicron变种已被证明具有高度传播性，并在世界许多地区超过了Delta变种。在这项研究中，研究人员研究了叙利亚金仓鼠的Omicron感染。尽管感染先前的SARS-CoV-2变异体导致仓鼠感染后体重显著减轻，但感染Omicron变异体并没有导致任何可检测的体重减轻，即使在高攻击剂量下也是如此。此外，与先前的SARS-CoV-2变体相比，Omicron感染导致较低的病毒载量和减少的肺部病理学。这些发现表明，与先前的SARS-CoV-2变体相比，SARS-CoV-2 Omicron变体导致仓鼠的下呼吸道疾病不太严重。与先前感染SARS-CoV-2变体的仓鼠相比，感染SARS-CoV-2 Omicron变体的叙利亚金仓鼠体重没有减轻，肺部病毒较少，肺部病理学减轻。这些观察结果与新出现的人类数据一致，表明Omicron感染的严重程度低于先前的变体感染。
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron (B.1.1.529) variant has proven to be highly transmissible and has outcompeted the Delta variant in many regions of the world. Early reports have also suggested that Omicron may result in less severe clinical disease in humans. Here, we show that Omicron is less pathogenic than prior SARS-CoV-2 variants in Syrian golden hamsters. Hamsters were inoculated with either SARS-CoV-2 Omicron or other SARS-CoV-2 variants. Animals were followed for weight loss, and upper and lower respiratory tract tissues were assessed for viral loads and histopathology. Infection of hamsters with the SARS-CoV-2 WA1/2020, Alpha, Beta, or Delta strains led to 4%–10% weight loss by day 4 and 10%–17% weight loss by day 6. In contrast, infection of hamsters with two different Omicron challenge stocks did not result in any detectable weight loss, even at high challenge doses. Omicron infection led to substantial viral replication in both the upper and lower respiratory tracts but demonstrated lower viral loads in lung parenchyma and reduced pulmonary pathology compared with WA1/2020 infection. These data suggest that the SARS-CoV-2 Omicron variant may result in robust upper respiratory tract infection, but less severe lower respiratory tract clinical disease, compared with prior SARS-CoV-2 variants. Funding for this study was provided by NIH grant CA260476, the Massachusetts Consortium for Pathogen Readiness, the Ragon Institute, and the Musk Foundation. The SARS-CoV-2 Omicron variant has proven highly transmissible and has outcompeted the Delta variant in many regions of the world. In this study, investigators studied Omicron infection in Syrian golden hamsters. Although infection with prior SARS-CoV-2 variants led to substantial weight loss following infection in hamsters, infection with the Omicron variant did not result in any detectable weight loss, even at high challenge doses. Moreover, Omicron infection led to lower viral loads and reduced pathology in the lung compared with prior SARS-CoV-2 variants. These findings show that the SARS-CoV-2 Omicron variant led to less severe lower respiratory tract disease compared with prior SARS-CoV-2 variants in hamsters. Syrian golden hamsters infected with the SARS-CoV-2 Omicron variant did not lose weight and showed less virus in the lung and reduced lung pathology compared with hamsters infected with prior SARS-CoV-2 variants. These observations are consistent with emerging human data suggesting that Omicron infection is less severe than infection with prior variants.
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影响因子：
5.4
作者：
Dagotto, Gabriel;Mercado, Noe B.;Barouch, Dan H.
通讯作者：
Barouch, Dan H.
影响因子：
56.9
作者：
Yu, Jingyou;Tostanoski, Lisa H.;Barouch, Dan H.
通讯作者：
Barouch, Dan H.
影响因子：
64.8
作者：
Shuai, Huiping;Chan, Jasper Fuk-Woo;Chu, Hin
通讯作者：
Chu, Hin
影响因子：
82.9
作者：
Tostanoski LH;Wegmann F;Martinot AJ;Loos C;McMahan K;Mercado NB;Yu J;Chan CN;Bondoc S;Starke CE;Nekorchuk M;Busman-Sahay K;Piedra-Mora C;Wrijil LM;Ducat S;Custers J;Atyeo C;Fischinger S;Burke JS;Feldman J;Hauser BM;Caradonna TM;Bondzie EA;Dagotto G;Gebre MS;Jacob-Dolan C;Lin Z;Mahrokhian SH;Nampanya F;Nityanandam R;Pessaint L;Porto M;Ali V;Benetiene D;Tevi K;Andersen H;Lewis MG;Schmidt AG;Lauffenburger DA;Alter G;Estes JD;Schuitemaker H;Zahn R;Barouch DH
通讯作者：
Barouch DH
影响因子：
64.8
作者：
Cele S;Jackson L;Khoury DS;Khan K;Moyo-Gwete T;Tegally H;San JE;Cromer D;Scheepers C;Amoako DG;Karim F;Bernstein M;Lustig G;Archary D;Smith M;Ganga Y;Jule Z;Reedoy K;Hwa SH;Giandhari J;Blackburn JM;Gosnell BI;Abdool Karim SS;Hanekom W;NGS-SA;COMMIT-KZN Team;von Gottberg A;Bhiman JN;Lessells RJ;Moosa MS;Davenport MP;de Oliveira T;Moore PL;Sigal A
通讯作者：
Sigal A