PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
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DOI：
10.1038/s41598-018-28188-1
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发表时间：
2018-07-06
影响因子：
4.6
通讯作者：
Friml J
中科院分区：
文献类型：
--
作者：
Grones P;Abas M;Hajný J;Jones A;Waidmann S;Kleine-Vehn J;Friml J
植物激素生长素的细胞间分布很大程度上取决于质膜 PIN 型 (PIN) 生长素转运蛋白的极性亚细胞分布。响应不同发育和环境信号的 PIN 极性切换已被证明可以重定向介导某些发育反应的生长素通量。不同位点和不同激酶的 PIN 磷酸化对于 PIN 功能至关重要。在这里，我们研究了 PIN 磷酸化在向地反应过程中的作用。 PINOID 和相关激酶中的功能丧失和获得功能突变体（但 D6PK 激酶中没有）以及模拟两个预测磷酸化位点簇的组成型去磷酸化或磷酸化状态的突变部分破坏了 PIN3 磷酸化，并导致根和下胚轴的向地弯曲缺陷。特别是，它们影响了 PIN3 极性重排，以响应重力和生长素本身的反馈调节。因此，PIN 磷酸化除了调节转运活性和顶端-基底靶向外，对于响应环境和内源信号的快速极性切换也很重要。
Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals.
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影响因子：
3.5
作者：
Dory M;Hatzimasoura E;Kállai BM;Nagy SK;Jäger K;Darula Z;Nádai TV;Mészáros T;López-Juez E;Barnabás B;Palme K;Bögre L;Ditengou FA;Dóczi R
通讯作者：
Dóczi R
影响因子：
27.5
作者：
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通讯作者：
Friml, Jiri
影响因子：
5.6
作者：
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通讯作者：
Cho HT
影响因子：
2.9
作者：
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通讯作者：
Luschnig C
影响因子：
9.8
作者：
Jia W;Li B;Li S;Liang Y;Wu X;Ma M;Wang J;Gao J;Cai Y;Zhang Y;Wang Y;Li J;Wang Y
通讯作者：
Wang Y