The quantification of gluconeogenesis in healthy men by (2)H2O and [2-(13)C]glycerol yields different results: rates of gluconeogenesis in healthy men measured with (2)H2O are higher than those measured with [2-(13)C]glycerol.
The quantification of gluconeogenesis in healthy men by (2)H2O and [2-(13)C]glycerol yields different results: rates of gluconeogenesis in healthy men measured with (2)H2O are higher than those measured with [2-(13)C]glycerol.
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通过 (2)H2O 和 [2-(13)C] 甘油对健康男性的糖异生进行定量会产生不同的结果：使用 (2)H2O 测量的健康男性的糖异生率高于使用 [2-(13) 测量的糖异生率
DOI：
10.1210/jcem.86.5.7383
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
J. Romijn
中科院分区：
文献类型：
--
作者：
M. Ackermans;A. Arias;P. Bisschop;P. Bisschop;E. Endert;H. Sauerwein;J. Romijn
(2)H2O 和 [2-(13)C] 甘油对糖异生 (GNG) 的定量以及葡萄糖的质量同位素稀释分析不涉及有关草酰乙酸前体库富集的假设。为了比较这两种方法，我们在相同的、严格标准化的真热量条件下测量了 6 名健康吸收后男性的 GNG，一次是口服 (2)H2O 后，一次是在启动的连续输注 [2-(13)C] 甘油期间。通过输注[6,6-(2)H(2)]葡萄糖来测量内源性葡萄糖产量(EGP)。 (2)H2O给药后或[2-(13)C]甘油输注期间EGP没有差异（12.2 +/- 0.7 vs. 11.7 +/- 0.3 micromol/kg.min）。然而，(2)H2O给药后测得的GNG显着高于[2-(13)C]甘油输注期间的GNG（7.4 +/- 0.7 vs. 4.9 +/- 0.6 micromol/kg.min；P = 0.03），分别约为EGP的60%和41%。 (2)H2O 研究在未标记甘油的预连续输注过程中重复进行，表明以 [2-(13)C] 甘油方法中使用的速率输注甘油不会影响 (2)H2O 的 GNG 测量，即。不含甘油时为 7.4 +/- 0.7，而含有甘油时为 7.6 +/- 0.9 micromol/kg.min，分别相当于 EGP 的大约 60% 和 62%。总之，通过 (2)H(2)O 测量的 GNG 比通过 [2-(13)C] 甘油测量的结果更高。这种差异不仅仅是由输注甘油本身引起的。相反，两种方法之间的差异可能与一种或两种方法中基本假设的概念问题有关。
The quantification of gluconeogenesis (GNG) by (2)H2O and [2-(13)C]glycerol and the mass isotopomer dilution analysis of glucose does not involve assumptions regarding the enrichment of the oxaloacetate precursor pool. To compare these two methods we measured GNG in six healthy postabsorptive males under identical, strictly standardized, eucaloric conditions, once after oral administration of (2)H2O and once during a primed continuous infusion of [2-(13)C]glycerol. Endogenous glucose production (EGP) was measured by infusion of [6,6-(2)H(2)]glucose. EGP was not different after (2)H2O administration or during [2-(13)C]glycerol infusion (12.2 +/- 0.7 vs. 11.7 +/- 0.3 micromol/kg.min). However, GNG measured after (2)H2O administration was significantly higher than that during [2-(13)C]glycerol infusion (7.4 +/- 0.7 vs. 4.9 +/- 0.6 micromol/kg.min; P = 0.03), representing approximately 60% and 41% of EGP, respectively. The (2)H2O study was repeated during primed continuous infusion of unlabeled glycerol, showing that infusion of glycerol at the rate used in the [2-(13)C]glycerol method does not affect the measurement of GNG with (2)H2O, viz. 7.4 +/- 0.7 without glycerol vs. 7.6 +/- 0.9 micromol/kg.min with glycerol, representing approximately 60% vs. 62% of EGP. In conclusion, GNG measured by (2)H(2)O yields higher results than those measured by [2-(13)C]glycerol. This discrepancy is not merely caused by infusion of glycerol per se. Rather, the discrepancy between both methods probably relates to conceptual problems in underlying assumptions in one or both methods.
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使用质量同位素异构体分布来测量禁食人类的糖异生作用的限制。
DOI：
10.1152/ajpendo.1995.269.1.e18
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Landau,BR;Fernandez,CA;Previs,SF;Ekberg,K;Chandramouli,V;Wahren,J;Kalhan,SC;Brunengraber,H
通讯作者：
Brunengraber,H
DOI：
10.1152/ajpendo.1996.270.1.e186
发表时间：
1996-01-01
影响因子：
5.1
作者：
Petersen, KF;Price, T;Shulman, GI
通讯作者：
Shulman, GI
正常人和糖尿病患者的葡萄糖产生、回收和糖异生：大规模同位素[U-13C]葡萄糖研究。
DOI：
10.1152/ajpendo.1996.270.4.e709
发表时间：
1996
期刊：
The American journal of physiology
影响因子：
--
作者：
Tayek,JA;Katz,J
通讯作者：
Katz,J
影响因子：
15.9
作者：
Landau, BR;Wahren, J;Kalhan, SC
通讯作者：
Kalhan, SC
影响因子：
15.9
作者：
JENSSEN, T;NURJHAN, N;GERICH, JE
通讯作者：
GERICH, JE