Syk-deficient eosinophils show normal interleukin-5-mediated differentiation, maturation, and survival but no longer respond to FcgammaR activation.
Syk-deficient eosinophils show normal interleukin-5-mediated differentiation, maturation, and survival but no longer respond to FcgammaR activation.
复制标题
Syk 缺陷的嗜酸性粒细胞表现出正常的白细胞介素 5 介导的分化、成熟和存活，但不再对 FcgammaR 激活做出反应。
作者：
E. Lach‐Trifilieff;K. Menear;E. Schweighoffer;V. Tybulewicz;C. Walker
酪氨酸激酶Syk在白细胞介素-5（IL-5）的抗嗜酸性粒细胞凋亡作用中起重要作用。然而，很少有人知道Syk参与其他IL-5介导的激活事件。为了进一步解决这些问题，Syk在IL-5诱导的嗜酸性粒细胞分化，活化和存活中的作用进行了分析，使用从Syk缺陷小鼠获得的细胞。我们可以证明Syk缺陷的胎肝细胞以与野生型胎肝细胞相同的速率响应于IL-5分化成成熟的嗜酸性粒细胞，并产生相同总数的嗜酸性粒细胞。此外，可以证明Syk（-/-）和野生型嗜酸性粒细胞之间IL-5诱导的成熟嗜酸性粒细胞存活率没有差异，这表明IL-5的抗凋亡作用不需要Syk，尽管IL-5受体连接后该酪氨酸激酶被激活。相比之下，Syk缺陷型小鼠而非野生型小鼠的嗜酸性粒细胞不能响应Fc γ受体（Fc γ R）接合而产生活性氧中间体。总之，这些数据清楚地表明Syk在IL-5介导的嗜酸性粒细胞分化或存活中没有关键作用，但强调了这种酪氨酸激酶在FcgammaR刺激诱导的活化事件中的重要性。
The tyrosine kinase Syk has been proposed to play a critical role in the antiapoptotic effect of interleukin (IL)-5 in human eosinophils. However, little is known about the involvement of Syk in other IL-5-mediated activation events. To further address these questions, the role of Syk in IL-5-induced eosinophil differentiation, activation, and survival was analyzed using cells obtained from Syk-deficient mice. We could demonstrate that Syk-deficient fetal liver cells differentiate into mature eosinophils in response to IL-5 at the same rate as wild-type fetal liver cells and generate the same total number of eosinophils. Moreover, no difference in IL-5-induced survival of mature eosinophils between Syk(-/-) and wild-type eosinophils could be demonstrated, suggesting that the antiapoptotic effect of IL-5 does not require Syk despite the activation of this tyrosine kinase upon IL-5 receptor ligation. In contrast, eosinophils derived from Syk-deficient but not wild-type mice were incapable of generating reactive oxygen intermediates in response to Fcgamma receptor (FcgammaR) engagement. Taken together, these data clearly demonstrate no critical role for Syk in IL-5-mediated eosinophil differentiation or survival but underline the importance of this tyrosine kinase in activation events induced by FcgammaR stimulation.
DOI：
10.1073/pnas.93.18.9704
发表时间：
1996-09-03
影响因子：
11.1
作者：
MallickWood, CA;Pao, W;Hayday, AC
通讯作者：
Hayday, AC
影响因子：
14.2
作者：
GLEICH, GJ
通讯作者：
GLEICH, GJ
与内皮细胞共培养的嗜酸性粒细胞具有增加的存活率和功能特性。
DOI：
10.1126/science.3110954
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rothenberg,ME;OwenJr,WF;Silberstein,DS;Soberman,RJ;Austen,KF;Stevens,RL
通讯作者：
Stevens,RL