Different Stages of Quiescence, Senescence, and Cell Stress Identified by Molecular Algorithm Based on the Expression of Ki67, RPS6, and Beta-Galactosidase Activity.
Different Stages of Quiescence, Senescence, and Cell Stress Identified by Molecular Algorithm Based on the Expression of Ki67, RPS6, and Beta-Galactosidase Activity.
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DOI：
10.3390/ijms22063102
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发表时间：
2021-03-18
影响因子：
5.6
通讯作者：
Galderisi U
中科院分区：
文献类型：
--
作者：
Alessio N;Aprile D;Cappabianca S;Peluso G;Di Bernardo G;Galderisi U
在其寿命期间，细胞有两种可能的状态：非循环静止状态（G 0）和循环激活状态。细胞可以进入可逆的静止G 0状态，或者，它们可以经历不可逆的G 0状态。后者可以是生理分化，或者在应激事件之后是衰老状态。几个G 0状态之间的歧视是一个重要的调查，因为静止，分化和衰老是渐进的现象与中间过渡阶段。我们使用Ki 67、RPS 6和β-半乳糖苷酶的表达来鉴定通过G 0进入、G 0和G 0警戒状态逐渐进入和离开静止期的健康细胞。然后，我们评估了细胞如何在遗传毒性应激事件后进入衰老。我们确定了一个初始的压力阶段与β-半乳糖苷酶和Ki 67增殖标志物的表达。细胞可以从应激事件中恢复或通过早期和晚期衰老状态而衰老。静止和衰老之间的区分是基于RPS 6的表达，RPS 6是存在于衰老细胞中但不存在于静止细胞中的活性蛋白质合成的标志物。即使考虑到固定的G 0状态不存在，我们的分子算法可能代表一种方法，用于识别G 0过渡状态的转折点，不断变化。
During their life span, cells have two possible states: a non-cycling, quiescent state (G0) and a cycling, activated state. Cells may enter a reversible G0 state of quiescence or, alternatively, they may undergo an irreversible G0 state. The latter may be a physiological differentiation or, following a stress event, a senescent status. Discrimination among the several G0 states represents a significant investigation, since quiescence, differentiation, and senescence are progressive phenomena with intermediate transitional stages. We used the expression of Ki67, RPS6, and beta-galactosidase to identify healthy cells that progressively enter and leave quiescence through G0-entry, G0 and G0-alert states. We then evaluated how cells may enter senescence following a genotoxic stressful event. We identified an initial stress stage with the expression of beta-galactosidase and Ki67 proliferation marker. Cells may recover from stress events or become senescent passing through early and late senescence states. Discrimination between quiescence and senescence was based on the expression of RPS6, a marker of active protein synthesis that is present in senescent cells but absent in quiescent cells. Even taking into account that fixed G0 states do not exist, our molecular algorithm may represent a method for identifying turning points of G0 transitional states that continuously change.
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DOI：
10.3233/bpl-160038
发表时间：
2017-11-09
期刊：
Brain plasticity (Amsterdam, Netherlands)
影响因子：
--
作者：
Baser A;Skabkin M;Martin-Villalba A
通讯作者：
Martin-Villalba A
DOI：
10.18632/aging.100443
发表时间：
2012-03
期刊：
Aging
影响因子：
--
作者：
Blagosklonny MV
通讯作者：
Blagosklonny MV
影响因子：
5.3
作者：
Sun, Xiaoming;Bizhanova, Aizhan;Kaufman, Paul D.
通讯作者：
Kaufman, Paul D.
影响因子：
3.1
作者：
Ishikawa, Kazuhiro;Ishii, Hideshi;Saito, Toshiyuki
通讯作者：
Saito, Toshiyuki
影响因子：
18.2
作者：
Campisi J
通讯作者：
Campisi J