The electrical resistance breakdown assay determines the role of proteinases in tumor cell invasion.
The electrical resistance breakdown assay determines the role of proteinases in tumor cell invasion.
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电阻击穿测定确定了蛋白酶在肿瘤细胞侵袭中的作用。
DOI：
10.1152/ajprenal.00327.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Schneider
中科院分区：
文献类型：
--
作者：
T. Ludwig;R. Ossig;S. Graessel;M. Wilhelmi;H. Oberleithner;S. Schneider
Madin-Darby犬肾（MDCK）细胞单层的电阻击穿提供了一个连续的癌症侵袭检测系统，在形态学改变之前检测功能变化。在这项研究中，我们解决的问题，肿瘤细胞和上皮细胞单层之间的物理接触是否是肿瘤细胞侵袭的先决条件。当直接接种人黑素瘤细胞时（即，物理接触）在电紧密的上皮细胞层（5，800 +/-106 Ω × cm 2）的顶部，电单层泄漏导致24小时内跨上皮电阻降低18 +/-3%。然而，当黑色素瘤细胞接种在上皮细胞单层的基底外侧表面附近但被滤膜隔开时（即，无物理接触），漏电发生得更快（24小时内减少42 +/- 3%）。原子力显微镜检测到细胞之间的离散结构变化。基质金属蛋白酶抑制剂α 2-巨球蛋白或伊洛马司他可有效阻止漏电。我们的结论是，可溶性蛋白酶的胞吐导致MDCK单层的电击穿，独立于肿瘤细胞和单层之间的物理接触。
The electrical resistance breakdown of the Madin-Darby canine kidney (MDCK) cell monolayer provides a continuous assay system for cancer invasion that detects functional changes before morphological alterations. In this study, we address the question of whether physical contact between tumor cell and epithelial monolayer is a prerequisite for tumor cell invasion. When human melanoma cells were seeded directly (i.e., physical contact) on top of an electrically tight epithelial cell layer (5,800 +/- 106 Omega x cm2), electrical monolayer leakage led to an 18 +/- 3% reduction of transepithelial electrical resistance within 24 h. However, when melanoma cells were seeded close to the basolateral surface of the epithelial cell monolayer but separated by a filter membrane (i.e., no physical contact), electrical leakage occurred even more quickly (42 +/- 3% reduction in 24 h). Atomic force microscopy detected discrete structural changes between cells. Electrical leakage was effectively blocked by alpha2-macroglobulin or ilomastat, inhibitors of matrix metalloproteinases. We conclude that exocytosis of soluble proteases causes electrical breakdown of the MDCK monolayer, independently of physical contact between tumor cells and the monolayer.
组织样品中基质金属蛋白酶的定量。
影响因子：
--
作者：
WoessnerJr,JF
通讯作者：
WoessnerJr,JF
影响因子：
56.9
作者：
CUNNINGHAM, CC;GORLIN, JB;STOSSEL, TP
通讯作者：
STOSSEL, TP
影响因子：
3.1
作者：
M. Stearns;Stearns Me
通讯作者：
M. Stearns;Stearns Me