Molecular detection and characterization of tick-borne pathogens in dogs and ticks from Nigeria.
Molecular detection and characterization of tick-borne pathogens in dogs and ticks from Nigeria.
复制标题
DOI：
10.1371/journal.pntd.0002108
复制
发表时间：
2013
影响因子：
3.8
通讯作者：
Harrus S
中科院分区：
文献类型：
--
作者：
Kamani J;Baneth G;Mumcuoglu KY;Waziri NE;Eyal O;Guthmann Y;Harrus S
目前关于尼日利亚狗和蜱中媒介传播和人畜共患病原体流行情况的信息有限。本研究的目的是利用分子技术在尼日利亚的狗和蜱中检测和表征媒介传播的病原体。采用聚合酶链反应（PCR）和测序方法对尼日利亚181只狗的血样和蜱（血根头蜱、图兰根头蜱和淋滤血蜱）进行了人畜媒介传播病原体分子筛选。检出犬肝虫（41.4%）、犬埃利希体（12.7%）、立克次体（8.8%）、罗西巴贝斯虫（6.6%）、platys无形体（6.6%）、vogeli巴贝斯虫（0.6%）、Theileria sp.（0.6%）。在181只犬中42只采集的258只蜱中检出犬伊螨（23.7%）、犬嗜血杆菌（21.1%）、立克次体（10.5%）、mikurenis新埃利希体（5.3%）和platys伊螨（1.9%）。同时感染两种病原体的犬只占37%，有一只犬同时感染3种病原体。在1只犬和1只血蜱中检出了以色列康氏立克次体DNA。在血根头蜱和淋血蜱中检测到另一种人类病原体米库奈德念珠菌的DNA，这是非洲首次发现米库奈德念珠菌。本研究在当地犬中检测到的伊氏大肠杆菌DNA序列与绵羊的卵形伊氏大肠杆菌序列同源性达98%。本研究结果表明，尼日利亚的狗及其蜱中存在大量人类和动物病原体，并表明人类暴露于这些病原体感染的潜在高风险。在尼日利亚，狗不仅被当作宠物饲养，而且还被用于狩猎，在一些民族中，狗是动物蛋白的来源。众所周知，蜱虫会感染狗，并作为一些人畜共患病和重要兽医病原体的载体。关于尼日利亚狗和蜱中媒介传播病原体的流行和分布的信息有限。该研究的目的是利用分子方法在尼日利亚的狗和蜱中检测和表征媒介传播的病原体。本研究结果显示，尼日利亚犬（77.3%）和蜱（63.3%）中媒介传播病原体的估计值较高。在血根头蜱和淋血蜱中检测到一种新出现的人类病原体mikurencandidatus N. DNA。在血根头蜱中检出地中海斑疹热的病原体以色列立克次体。这是对非洲的米库伦候选体和尼日利亚的以色列康氏立克次体的首次描述。这些结果表明，在发展中国家使用分子技术诊断新发感染对于帮助医生和兽医做出准确诊断并为患者提供适当治疗至关重要。
Only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in Nigeria. The aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from Nigeria. Blood samples and ticks (Rhipicephalus sanguineus, Rhipicephalus turanicus and Heamaphysalis leachi) collected from 181 dogs from Nigeria were molecularly screened for human and animal vector-borne pathogens by PCR and sequencing. DNA of Hepatozoon canis (41.4%), Ehrlichia canis (12.7%), Rickettsia spp. (8.8%), Babesia rossi (6.6%), Anaplasma platys (6.6%), Babesia vogeli (0.6%) and Theileria sp. (0.6%) was detected in the blood samples. DNA of E. canis (23.7%), H. canis (21.1%), Rickettsia spp. (10.5%), Candidatus Neoehrlichia mikurensis (5.3%) and A. platys (1.9%) was detected in 258 ticks collected from 42 of the 181 dogs. Co- infections with two pathogens were present in 37% of the dogs examined and one dog was co-infected with 3 pathogens. DNA of Rickettsia conorii israelensis was detected in one dog and Rhipicephalus sanguineus tick. DNA of another human pathogen, Candidatus N. mikurensis was detected in Rhipicephalus sanguineus and Heamaphysalis leachi ticks, and is the first description of Candidatus N. mikurensis in Africa. The Theileria sp. DNA detected in a local dog in this study had 98% sequence identity to Theileria ovis from sheep. The results of this study indicate that human and animal pathogens are abundant in dogs and their ticks in Nigeria and portray the potential high risk of human exposure to infection with these agents. In Nigeria, dogs are not only kept as pets, but are also used for hunting as well as a source of animal protein among some ethnic groups. Ticks are known to infest dogs and serve as vectors for some pathogens of zoonotic and veterinary importance. There is limited information on the prevalence and distribution of vector borne pathogens in dogs and ticks in Nigeria. The aim of the study was to detect and characterize vector borne pathogens in dogs and ticks from Nigeria using molecular methods. The results of this study showed a high estimate of vector borne pathogens in Nigerian dogs (77.3%) and ticks (63.3%). DNA of Candidatus N. mikurensis, an emerging pathogen of humans was detected in Rhipicephalus sanguineus and Heamaphysalis leachi ticks. Another human pathogen, Rickettsia conorii israelensis the causative agent of Mediterranean spotted fever was detected in Rhipicephalus sanguineus ticks. This is the first description of Candidatus N. mikurensis in Africa and Rickettsia conorii israelensis in Nigeria. These results indicate that the use of molecular techniques for the diagnosis of emerging infections in developing countries is of utmost importance in assisting physicians and veterinarians in making accurate diagnoses and providing the appropriate treatment for their patients.
登录
查看更多内容
影响因子：
3.2
作者：
Cardoso L;Yisaschar-Mekuzas Y;Rodrigues FT;Costa A;Machado J;Diz-Lopes D;Baneth G
通讯作者：
Baneth G
DOI：
10.1128/cdli.12.11.1343-1346.2005
发表时间：
2005-11-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Oyamada, M;Davoust, B;Inokuma, H
通讯作者：
Inokuma, H
影响因子：
2.6
作者：
Peleg, Ofer;Baneth, Gad;Harrus, Shirnon
通讯作者：
Harrus, Shirnon
影响因子：
2.7
作者：
Olmeda, AS;Armstrong, PM;Telford, SR
通讯作者：
Telford, SR
影响因子：
2.1
作者：
Foongladda, Suporn;Inthawong, Dutsadee;Gaywee, Jariyanart
通讯作者：
Gaywee, Jariyanart