Lipopolysaccharide and platelet-activating factor stimulate expression of platelet-activating factor acetylhydrolase via distinct signaling pathways.
Lipopolysaccharide and platelet-activating factor stimulate expression of platelet-activating factor acetylhydrolase via distinct signaling pathways.
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DOI：
10.1007/s00011-011-0326-5
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发表时间：
2011-08
影响因子：
6.7
通讯作者：
Patel, Nipa
中科院分区：
文献类型：
--
作者：
Howard, Katherine M.;Abdel-al, Mohammed;Ditmyer, Marcia;Patel, Nipa
本研究旨在探讨血小板活化因子（PAF）诱导血小板活化因子乙酰水解酶（PAF-AH）表达的能力。应用核糖核酸酶保护试验和实时荧光定量PCR技术研究了脂多糖（LPS）和PAF对人单核巨噬细胞6（MM 6）细胞PAF-AH mRNA表达的调节作用。用丝裂原活化蛋白激酶（MAPK）抑制剂和PAF受体拮抗剂研究了PAF-AH的调节机制。LPS或PAF均能使PAF-AH mRNA表达量呈剂量依赖性增加。LPS诱导的PAF-AH表达比PAF刺激的反应更有效和快速增加。然而，当同时给药时，PAF增强了LPS刺激的反应。LPS刺激的PAF-AH表达易受p38 MAPK抑制剂和PAF受体拮抗剂的部分抑制。PAF诱导的PAF-AH水平上调仅通过PAF受体介导，并且不依赖于p38 MAPK。促炎介质LPS和PAF通过不同的信号通路增加PAF-AH mRNA的水平。
This study was designed to investigate and characterize the ability of platelet-activating factor (PAF) to induce the expression of platelet-activating factor acetylhydrolase (PAF-AH). Ribonuclease protection assays and quantitative real-time PCR were used to investigate the ability of lipopolysaccharide (LPS) and PAF to regulate PAF-AH mRNA expression in human monocyte–macrophage 6 (MM6) cells. Pharmacological inhibitors of mitogen activated protein kinases (MAPK) and PAF receptor antagonists were used to investigate the mechanism of regulation of PAF-AH. PAF-AH mRNA levels were increased upon exposure to LPS or PAF in a dose-dependent manner. LPS elicited a more potent and rapid increase in PAF-AH expression than the PAF-stimulated response. However, when administered concomitantly, PAF augmented the LPS-stimulated response. LPS-stimulated PAF-AH expression was susceptible to partial inhibition by a p38 MAPK inhibitor and PAF receptor antagonists. PAF-induced up-regulation of PAF-AH levels was solely mediated via the PAF receptor and was p38 MAPK-independent. The proinflammatory mediators, LPS and PAF, increased levels of PAF-AH mRNA via distinct signaling pathways.
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影响因子：
64.8
作者：
TJOELKER, LW;WILDER, C;GRAY, PW
通讯作者：
GRAY, PW
影响因子：
4.8
作者：
Howard, KM;Olson, MS
通讯作者：
Olson, MS
影响因子：
5.1
作者：
Zipfel, A;Schenk, M;Viebahn, R
通讯作者：
Viebahn, R
影响因子：
4.4
作者：
Jungi, TW;Brcic, M;Eperon, S
通讯作者：
Eperon, S
DOI：
10.1016/0002-9378(93)90614-o
发表时间：
1993-09-01
影响因子：
9.8
作者：
NARAHARA, H;JOHNSTON, JM
通讯作者：
JOHNSTON, JM