Neuroproteomic study of nitrated proteins in moderate traumatic brain injured rats treated with gamma glutamyl cysteine ethyl ester administration post injury: Insight into the role of glutathione elevation in nitrosative stress.
Neuroproteomic study of nitrated proteins in moderate traumatic brain injured rats treated with gamma glutamyl cysteine ethyl ester administration post injury: Insight into the role of glutathione elevation in nitrosative stress.
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DOI：
10.1002/prca.201600004
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Reed TT
中科院分区：
文献类型：
--
作者：
Henderson M;Rice B;Sebastian A;Sullivan PG;King C;Robinson RA;Reed TT
本研究的目的是建立一个时间点，以确定事件后施用 GCEE 以减少氧化损伤的最有利时间，其次，通过使用氧化还原蛋白质组学，确定 GCEE 是否可以轻松抑制 TBI 动物中的 3-NT 修饰。通过使用 Wistar 大鼠的中度创伤性脑损伤模型，假设 3-硝基酪氨酸 (3-NT) 形成作为中间体的作用将预测大脑中蛋白质硝化/亚硝化和氧化损伤的参与。在该实验中，与遭受损伤并接受 150 mg/kg 谷胱甘肽模拟物 GCEE 治疗的大鼠相比，经 TBI 损伤、经盐水治疗的大鼠中蛋白质羰基、4-羟基壬烯醛和 3-硝基酪氨酸的水平显着升高。确定3-NT水平升高的存在可以深入了解蛋白质硝化/亚硝化与氧化损伤之间的关系，从而可以确定特定神经系统疾病的发病机制和进展。
The aims of this study are to establish a time point to determine the most beneficial time to administer GCEE post incident to reduce oxidative damage and second, by using redox proteomics, to determine if GCEE can readily suppress 3-NT modification in TBI animals. By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that the role of 3-nitrotyrosine (3-NT) formation as an intermediate will predict the involvement of protein nitration/nitrosation and oxidative damage in the brain. In this experiment, the levels of protein carbonyls, 4-hydroxynonenal, and 3-nitrotyrosine were significantly elevated in TBI injured, saline treated rats compared with those who sustained an injury and were treated with 150 mg/kg of the glutathione mimetic, GCEE. Determining the existence of elevated 3-NT levels provides insight into the relationship between the protein nitration/nitrosation and the oxidative damage, which can determine the pathogenesis and progression of specific neurological diseases.
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