Features of mammalian microRNA promoters emerge from polymerase II chromatin immunoprecipitation data.
Features of mammalian microRNA promoters emerge from polymerase II chromatin immunoprecipitation data.
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DOI：
10.1371/journal.pone.0005279
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Benos PV
中科院分区：
文献类型：
--
作者：
Corcoran DL;Pandit KV;Gordon B;Bhattacharjee A;Kaminski N;Benos PV
MicroRNAs(MiRNAs)是蛋白质编码基因的短的、非编码的RNA调节因子。MiRNAs在多种生物学过程和各种疾病中发挥着非常重要的作用。许多算法都能够预测miRNA基因及其靶点，但它们的转录调控仍在研究中。一般认为，基因内miRNAs(位于蛋白质编码基因的内含子或外显子)与宿主基因共转录，而大多数基因间miRNAs从各自的RNA聚合酶II(Pol II)启动子转录而来。然而，初级转录本和启动子组织的长度目前尚不清楚。我们使用已知miRNA基因区域周围的定制阵列进行了POL II染色质免疫沉淀(CHIP)芯片。为了确定miRNA基因的真正核心转录起始点，我们开发了一种新的工具(CPPP)。我们发现miRNA基因可以从几千个碱基以外的启动子转录，并且它们的启动子具有与蛋白质编码基因相同的一般特征。最后，我们发现有证据表明，多达26%的基因内miRNAs可能是从它们自己独特的启动子转录而来的。MiRNA启动子具有与蛋白质编码基因相似的特征，但miRNA转录组织更为复杂。
MicroRNAs (miRNAs) are short, non-coding RNA regulators of protein coding genes. miRNAs play a very important role in diverse biological processes and various diseases. Many algorithms are able to predict miRNA genes and their targets, but their transcription regulation is still under investigation. It is generally believed that intragenic miRNAs (located in introns or exons of protein coding genes) are co-transcribed with their host genes and most intergenic miRNAs transcribed from their own RNA polymerase II (Pol II) promoter. However, the length of the primary transcripts and promoter organization is currently unknown. We performed Pol II chromatin immunoprecipitation (ChIP)-chip using a custom array surrounding regions of known miRNA genes. To identify the true core transcription start sites of the miRNA genes we developed a new tool (CPPP). We showed that miRNA genes can be transcribed from promoters located several kilobases away and that their promoters share the same general features as those of protein coding genes. Finally, we found evidence that as many as 26% of the intragenic miRNAs may be transcribed from their own unique promoters. miRNA promoters have similar features to those of protein coding genes, but miRNA transcript organization is more complex.
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影响因子：
56.9
作者：
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通讯作者：
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DOI：
10.1073/pnas.97.1.262
发表时间：
2000-01-04
影响因子：
11.1
作者：
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通讯作者：
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DOI：
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发表时间：
2000-08-29
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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