Genetic potential for disease resistance in critically endangered amphibians decimated by chytridiomycosis
Genetic potential for disease resistance in critically endangered amphibians decimated by chytridiomycosis
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因壶菌病而大量死亡的极度濒危两栖动物的抗病遗传潜力
DOI：
10.1111/acv.12459
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发表时间：
2018
影响因子：
3.4
通讯作者：
L. F. Skerratt
中科院分区：
文献类型：
--
作者：
T. A. Kosch; C. N. S. Silva; L. A. Brannelly; A. A. Roberts; Q. Lau; G. Marantelli ;L. Berger; L. F. Skerratt
在两栖类真菌病原菌Batrachochytrium dendrobatium（Bd）出现后，全世界数百种两栖类物种减少。尽管进行了圈养繁殖，但许多物种的野生种群不太可能重新建立，除非抗药性增加。我们在功能灭绝的南方科氏蛙Pseudophryne corroboree中进行了Bd挑战研究，以调查个体和种群之间Bd易感性的差异，识别与Bd抗性相关的遗传[主要组织相容性复合体（MHC）I类]和全基因组变异，并测量遗传多样性和种群遗传结构。我们发现三个MHC变异体和一个MHC超型与B感染负荷和存活沿着一个显著相关的单核苷酸多态性。我们还发现全基因组杂合性与生存率增加相关。此外，我们还发现在所研究的四个加州杨种群中存在显著的种群结构和较高的MHC遗传多样性。我们的研究结果表明，有圈养南方corroboree青蛙之间的免疫遗传差异，这种差异可以被操纵，以增加抗病能力，减轻壶菌病的重大威胁。这些结果证明了一种潜在的长期解决壶菌病的方法，可能包括培育更具抗性的个体并将其放归野外。
Hundreds of amphibian species have declined worldwide after the emergence of the amphibian fungal pathogenBatrachochytrium dendrobatidis(Bd). Despite captive breeding efforts, it is unlikely that wild populations for many species will be reestablished unlessBdresistance increases. We performed aBd‐challenge study in the functionally extinct southern corroboree frogPseudophryne corroboreeto investigate differences inBdsusceptibility among individuals and populations, identify genetic [major histocompatibility complex (MHC) class I] and genome‐wide variants associated withBdresistance, and measure genetic diversity and population genetic structure. We found three MHC variants and one MHC supertype associated withBdinfection load and survival along with a suggestively associated single nucleotide polymorphism. We also showed that genome‐wide heterozygosity is associated with increased survival. Additionally, we found evidence of significant population structure among the fourP. corroboreepopulations studied and high MHC genetic diversity. Our results indicate that there are immunogenetic differences among captive southern corroboree frogs; such differences could be manipulated to increase disease resistance and mitigate the significant threat of chytridiomycosis. These results demonstrate a potential long‐term solution to chytridiomycosis that could include breeding more resistant individuals and returning them to the wild.
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影响因子：
3.3
作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
通讯作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
影响因子：
4.1
作者：
Harrisson KA;Pavlova A;Telonis-Scott M;Sunnucks P
通讯作者：
Sunnucks P
影响因子：
4.4
作者：
Lal MM;Southgate PC;Jerry DR;Bosserelle C;Zenger KR
通讯作者：
Zenger KR
影响因子：
3.3
作者：
Brannelly, Laura A.;Webb, Rebecca J.;Berger, Lee
通讯作者：
Berger, Lee
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro