Alcohol-specific transcriptional dynamics of memory reconsolidation and relapse.
Alcohol-specific transcriptional dynamics of memory reconsolidation and relapse.
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DOI：
10.1038/s41398-023-02352-2
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发表时间：
2023-02-15
影响因子：
6.8
通讯作者：
Barak, Segev
中科院分区：
文献类型：
--
作者：
Goltseker, Koral;Garay, Patricia;Bonefas, Katherine;Iwase, Shigeki;Barak, Segev
复发是酒精成瘾的一个关键问题，可以通过破坏与酒精相关的记忆来减弱。记忆被认为在重新巩固过程中暂时不稳定。在这里，我们提供的证据，独特的转录动力学基础酒精记忆再巩固。使用小鼠的位置调节程序，我们发现，酒精记忆检索增加即刻早期基因在背海马和内侧前额叶皮层的mRNA表达，和酒精寻求废除检索后非特异性抑制基因转录，或下调ARC表达使用反义寡核苷酸。然而，由于恢复记忆的自然奖励（蔗糖）也增加了相同的立即早期基因表达，我们使用RNA测序探索酒精特异性转录变化。我们揭示了一个独特的转录指纹激活酒精记忆，这套可塑性相关基因的表达没有改变蔗糖记忆检索。我们的研究结果表明，酒精记忆可能会激活两个平行的转录程序：一个是参与记忆再巩固一般，另一个是专门激活酒精记忆过程中。
Relapse, a critical issue in alcohol addiction, can be attenuated by disruption of alcohol-associated memories. Memories are thought to temporarily destabilize upon retrieval during the reconsolidation process. Here, we provide evidence for unique transcriptional dynamics underpinning alcohol memory reconsolidation. Using a mouse place-conditioning procedure, we show that alcohol-memory retrieval increases the mRNA expression of immediate-early genes in the dorsal hippocampus and medial prefrontal cortex, and that alcohol seeking is abolished by post-retrieval non-specific inhibition of gene transcription, or by downregulating ARC expression using antisense-oligodeoxynucleotides. However, since retrieval of memories for a natural reward (sucrose) also increased the same immediate-early gene expression, we explored for alcohol-specific transcriptional changes using RNA-sequencing. We revealed a unique transcriptional fingerprint activated by alcohol memories, as the expression of this set of plasticity-related genes was not altered by sucrose-memory retrieval. Our results suggest that alcohol memories may activate two parallel transcription programs: one is involved in memory reconsolidation in general, and another is specifically activated during alcohol-memory processing.
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影响因子：
3.4
作者：
Exton-McGuinness MT;Lee JL
通讯作者：
Lee JL
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Antoine, Besnard;Serge, Laroche;Jocelyne, Caboche
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Jocelyne, Caboche
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作者：
Baunez, C;Dias, C;Amalric, M
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Amalric, M
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Hope BT
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作者：
Collins, Pamela Y.;Patel, Vikram;Joestl, Sarah S.;March, Dana;Insel, Thomas R.;Daar, Abdallah S.
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Daar, Abdallah S.