Mycorrhization of Quercus mongolica seedlings by Tuber melanosporum alters root carbon exudation and rhizosphere bacterial communities
Mycorrhization of Quercus mongolica seedlings by Tuber melanosporum alters root carbon exudation and rhizosphere bacterial communities
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黑孢块菌对蒙古栎幼苗的菌根改变了根部碳渗出和根际细菌群落
DOI：
10.1007/s11104-021-05112-7
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发表时间：
2021-04
期刊：
影响因子：
4.9
通讯作者：
Yu Fuqiang
中科院分区：
文献类型：
--
作者：
Wang Yanliang;Wang Ran;Lu Bin;Guerin-Laguette Alexis;He Xinhua;Yu Fuqiang
研究外生菌根 (ECM) 如何通过改变生理特征和根碳渗出来调节植物性能和根际土壤细菌群落。黑孢块菌定殖和未定殖的蒙古栎幼苗在由 41% 泥炭、41% 浮石、9% 松树皮和 9% 石灰组成的基质上生长。采用气体交换荧光系统、电感耦合等离子体原子发射光谱仪、高效液相色谱、气相色谱和质谱以及16S rRNA测序分析光合和营养特性、根际碳渗出物和细菌群落。块茎黑孢子菌根增加了根际提取物中的叶片光合速率（69%）、磷浓度（94%）、根际pH（0.4单位）、根际酸性磷酸酶活性（33%）和总有机碳（76%），但降低了叶片钾浓度（26%）和根际有机阴离子（50%）。此外，包括半乳糖在内的糖存在于定殖的幼苗的根际提取物中，但未定殖的幼苗中不存在。菌根化改变了根际细菌群落，定殖和未定殖的幼苗之间仅共享 1~10% 的操作分类单位 (OTU)； T. melanosporum 定殖的植物富含放线菌。受黑孢子菌定植影响的其他细菌 OTU 的差异丰度也与植物生理和/或根际因素的变化相关。我们的结果表明，T. melanosporum ECM 定殖可以调节在先前灭菌的泥炭基质中生长的蒙古栎幼苗的碳经济和根际细菌群落，以促进植物生长和养分循环。
To study how ectomycorrhizas (ECMs) mediate plant performance and rhizosphere soil bacterial communities via altered physiological characteristics and root carbon exudation. Tuber melanosporum-colonized and uncolonized Quercus mongolica seedlings were grown on a substrate consisting of 41% peat, 41% pumice, 9% pine bark and 9% lime. Gas exchange fluorescence system, inductively coupled plasma atomic-emission spectrometer, high-performance liquid chromatography, gas chromatography and mass spectrometry, and 16S rRNA sequencing were used to analyze photosynthetic and nutritional characteristics, rhizosphere carbon exudates, and bacterial communities. Tuber melanosporum mycorrhization increased leaf photosynthetic rate (69%), phosphorus concentration (94%), rhizosphere pH (0.4 units), rhizosphere acid phosphatase activity (33%) and total organic carbon (76%) in rhizosphere extracts but decreased leaf potassium concentration (26%) and rhizosphere organic anions (50%). Additionally, sugars including galactose were present in rhizosphere extract of colonized, but not uncolonized seedlings. Mycorrhization altered rhizosphere bacterial communities, with only ~ 10% operational taxonomic units (OTUs) shared between colonized and uncolonized seedlings; T. melanosporum colonized plants were enriched in actinobacteria. The differential abundances of other bacterial OTUs affected by T. melanosporum colonization were also correlated with variation in plant physiological and/or rhizosphere factors. Our results suggest that T. melanosporum ECM colonization may regulate carbon economy and rhizosphere bacterial communities of Q. mongolica seedlings grown in a previously sterilized peat-based substrate, to promote plant growth and nutrient cycling.
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