Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation.
Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation.
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DOI：
10.1038/ncomms3792
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发表时间：
2013
影响因子：
16.6
通讯作者：
Nishizawa, Naoko K.
中科院分区：
文献类型：
--
作者：
Kobayashi, Takanori;Nagasaka, Seiji;Senoura, Takeshi;Itai, Reiko Nakanishi;Nakanishi, Hiromi;Nishizawa, Naoko K.
铁是大多数生物所必需的。植物在铁利用率低的条件下转录诱导铁获取相关基因，但缺铁信号及其感应器的性质尚不清楚。在这里，我们报告了在水稻中发现的新的铁调节因子，命名为Oryza sativa Hamerytrin基序-包含真正有趣的新基因(RING)-以及锌指蛋白1(OsHRZ1)和OsHRZ2。OsHRZ1、OsHRZ2和它们的拟南芥同系物Brutus结合铁和锌，并具有泛素化活性。OsHRZ1和OsHRZ2在根中容易降解，与铁的条件无关。OsHRZ基因敲除植物对缺铁表现出很强的耐受性，无论土壤铁条件如何，都会在它们的地上部和籽粒中积累更多的铁。在OsHRZ基因敲除的植物中，涉及铁利用的缺铁诱导基因的表达增强，主要是在铁充足的条件下。这些结果表明，OsHRZ1和OsHRZ2是铁结合的感受器，在铁充足的情况下负向调节铁的获取。植物在低铁条件下激活基因转录反应，但它们如何感觉到铁水平不足尚不清楚。在这项研究中，小林等人。鉴定两种铁结合蛋白，它们具有泛素连接酶活性，并且是缺铁反应的负调节因子。
Iron is essential for most living organisms. Plants transcriptionally induce genes involved in iron acquisition under conditions of low iron availability, but the nature of the deficiency signal and its sensors are unknown. Here we report the identification of new iron regulators in rice, designated Oryza sativa Haemerythrin motif-containing Really Interesting New Gene (RING)- and Zinc-finger protein 1 (OsHRZ1) and OsHRZ2. OsHRZ1, OsHRZ2 and their Arabidopsis homologue BRUTUS bind iron and zinc, and possess ubiquitination activity. OsHRZ1 and OsHRZ2 are susceptible to degradation in roots irrespective of iron conditions. OsHRZ-knockdown plants exhibit substantial tolerance to iron deficiency, and accumulate more iron in their shoots and grains irrespective of soil iron conditions. The expression of iron deficiency-inducible genes involved in iron utilization is enhanced in OsHRZ-knockdown plants, mostly under iron-sufficient conditions. These results suggest that OsHRZ1 and OsHRZ2 are iron-binding sensors that negatively regulate iron acquisition under conditions of iron sufficiency. Plants activate a gene transcription response under low iron conditions but how they sense insufficient iron levels is unclear. In this study, Kobayashi et al. identify two iron-binding proteins that possess ubiquitin ligase activity and are negative regulators of the iron deficiency response.
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影响因子：
5.6
作者：
Masuda H;Kobayashi T;Ishimaru Y;Takahashi M;Aung MS;Nakanishi H;Mori S;Nishizawa NK
通讯作者：
Nishizawa NK
影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
4.3
作者：
Kobayashi, T;Nakanishi, H;Mori, S
通讯作者：
Mori, S
影响因子：
7.2
作者：
Kobayashi, Takanori;Itai, Reiko Nakanishi;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.
DOI：
10.1073/pnas.0707010104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Kobayashi, Takanori;Ogo, Yuko;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.