Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting.
Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting.
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DOI：
10.1267/ahc.17028
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发表时间：
2017-12-26
影响因子：
2.4
通讯作者：
Sakai N
中科院分区：
文献类型：
--
作者：
Shirafuji T;Ueyama T;Tanaka S;Hide I;Saito N;Sakai N
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There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively.
影响因子：
5.3
作者：
Rhodes, Kenneth J.;Trimmer, James S.
通讯作者：
Trimmer, James S.
DOI：
10.1369/0022155414545224
发表时间：
2014-10
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Hewitt SM;Baskin DG;Frevert CW;Stahl WL;Rosa-Molinar E
通讯作者：
Rosa-Molinar E
影响因子：
3.2
作者：
Couchman, John R.
通讯作者：
Couchman, John R.