Controls for immunohistochemistry: the Histochemical Society's standards of practice for validation of immunohistochemical assays.
Controls for immunohistochemistry: the Histochemical Society's standards of practice for validation of immunohistochemical assays.
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DOI：
10.1369/0022155414545224
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Rosa-Molinar E
中科院分区：
文献类型：
--
作者：
Hewitt SM;Baskin DG;Frevert CW;Stahl WL;Rosa-Molinar E
免疫组织化学广泛应用于生物医学研究，用于定位细胞和组织中分子的特定表位。基于免疫组织化学的解释的有效性需要适当的阳性和阴性对照，而这些通常在出版物中没有报道。这种遗漏可能会导致文献中的错误解释和不可复制的结果，并导致浪费时间、精力和资源，以及普通公众、立法机构和资助机构对科学调查的信心受到侵蚀。本文总结了确认免疫组织化学结果所需的基本控制措施，并代表了免疫组织化学在研究和诊断性调查中使用的实践标准。遵循本文中描述的指南可被作者引用，以支持其出版物中所报道的免疫组织化学解释的有效性。
Immunohistochemistry is widely used in biomedical research to localize specific epitopes of molecules in cells and tissues. The validity of interpretations based on immunohistochemistry requires appropriate positive and negative controls that are often not reported in publications. This omission may lead to incorrect interpretations and irreproducible results in the literature and contribute to wasted time, effort, and resources as well as erosion of confidence in scientific investigation by the general public, legislative bodies and funding agencies. The present article summarizes essential controls required for validation of immunohistochemical findings and represents a standard of practice for the use of immunohistochemistry in research and diagnostic investigations. Adherence to the guidelines described in the present article can be cited by authors as support for the validity of interpretations of the immunohistochemistry reported in their publications.
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影响因子：
3.2
作者：
Saper, Clifford B.
通讯作者：
Saper, Clifford B.
DOI：
10.1369/0022155414530995
发表时间：
2014-06
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Marchalant Y;Brownjohn PW;Bonnet A;Kleffmann T;Ashton JC
通讯作者：
Ashton JC
影响因子：
3.2
作者：
Burry, RW
通讯作者：
Burry, RW
影响因子：
64.8
作者：
Collins, Francis S.;Tabak, Lawrence A.
通讯作者：
Tabak, Lawrence A.
影响因子：
3.2
作者：
Holmseth, Silvia;Zhou, Yun;Danbolt, Niels Christian
通讯作者：
Danbolt, Niels Christian