Behavior of bacteriome symbionts during transovarial transmission and development of the Asian citrus psyllid.
Behavior of bacteriome symbionts during transovarial transmission and development of the Asian citrus psyllid.
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DOI：
10.1371/journal.pone.0189779
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nakabachi A
中科院分区：
文献类型：
--
作者：
Dan H;Ikeda N;Fujikami M;Nakabachi A
亚洲柑橘木虱（半翅目：柑橘木虱科）是一种世界性的严重害虫，它传播的是一种破坏性柑橘疾病黄龙病或黄龙病的病原体——自由候选菌（α变形杆菌）。在一个被称为细菌组的共生器官中，柑橘有一个类似细胞器的防御性共生体，Candidatus Profftella armatura (Betaproteobacteria)，和一个营养共生体，Ca. Carsonella ruddii （Gammaproteobacteria）。大量减少的共生体基因组和它们之间的代谢互补性表明它们之间的联系是不可缺少的。此外，Profftella和Liberibacter谱系之间的水平基因转移表明细菌群共生体与HLB病原体之间存在生态和进化相互作用。利用荧光原位杂交技术，研究了Profftella和Carsonella在柑桔经卵巢传播和发育过程中的行为。在性成熟的雌性成虫的细菌群中，共生体从极细长的管状形态转变为球形或短杆状形态，并向卵巢迁移。共生体然后形成马赛克团块，进入卵黄细胞的后极。缝合后，Carsonella和Profftella分别迁移到肿块的中央和周围。随着宿主细胞核的出现，团块细胞化，分别在中央合胞体和周围无核细菌细胞中分离出Carsonella和Profftella。随后，含有Profftella的单核细菌细胞聚集在后极，而含有Carsonella的合胞体位于前面，面向菌带，导致katatressis。在背侧闭合过程中，合胞体分裂成单核细菌细胞，包围着含有Profftella的细菌细胞团。一旦被完全包围，含有Profftella的细菌细胞团被融合成合胞体。在孵化之前，一对翅膀状的突出物从菌群的两侧产生，并在整个若虫期继续生长。这些发现为更好地理解柑橘及其微生物群之间的复杂关系提供了基础。
The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a serious pest worldwide, transmitting Candidatus Liberibacter spp. (Alphaproteobacteria), the causative agents of a devastating citrus disease known as huanglongbing or greening disease. In a symbiotic organ called the bacteriome, D. citri possesses an organelle-like defensive symbiont, Candidatus Profftella armatura (Betaproteobacteria), and a nutritional symbiont, Ca. Carsonella ruddii (Gammaproteobacteria). Drastically reduced symbiont genomes and metabolic complementarity among the symbionts and D. citri indicate their mutually indispensable association. Moreover, horizontal gene transfer between the Profftella and Liberibacter lineages suggests ecological and evolutionary interactions between the bacteriome symbiont and the HLB pathogen. Using fluorescence in situ hybridization, we examined the behavior of Profftella and Carsonella during transovarial transmission and the development of D. citri. In the bacteriomes of sexually-mature female adults, symbionts transformed from an extremely elongated tubular form into spherical or short-rod forms, which migrated toward the ovary. The symbionts then formed mosaic masses, which entered at the posterior pole of the vitellogenic oocytes. After anatrepsis, Carsonella and Profftella migrated to the central and peripheral parts of the mass, respectively. Following the appearance of host nuclei, the mass cellularized, segregating Carsonella and Profftella in the central syncytium and peripheral uninucleate bacteriocytes, respectively. Subsequently, the uninucleate bacteriocytes harboring Profftella assembled at the posterior pole, while the syncytium, containing Carsonella, sat on the anterior side facing the germ band initiating katatrepsis. During dorsal closure, the syncytium was divided into uninuclear bacteriocytes, which surrounded the mass of bacteriocytes containing Profftella. Once fully surrounded, the bacteriocyte mass containing Profftella was fused into a syncytium. Prior to hatching, a pair of wing-like protrusions arose from both lateral sides of the bacteriome, which continued to grow throughout the nymphal stages. These findings provide a foundation for better understanding the intricate relationship between D. citri and its microbiota.
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影响因子：
5.1
作者：
Kuechler, Stefan Martin;Gibbs, George;Hartung, Viktor
通讯作者：
Hartung, Viktor
影响因子：
64.8
作者：
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通讯作者：
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DOI：
10.1073/pnas.0800072105
发表时间：
2008-03-11
影响因子：
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影响因子：
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作者：
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