Three-dimensional X-ray visualization of axonal tracts in mouse brain hemisphere.
Three-dimensional X-ray visualization of axonal tracts in mouse brain hemisphere.
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DOI：
10.1038/srep35061
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发表时间：
2016-10-11
影响因子：
4.6
通讯作者：
Uesugi K
中科院分区：
文献类型：
--
作者：
Mizutani R;Saiga R;Ohtsuka M;Miura H;Hoshino M;Takeuchi A;Uesugi K
神经元通过轴突传递活性电位，这对大脑的功能至关重要。在这项研究中，用X射线断层扫描显微镜（也称为X射线显微断层扫描或微型CT）观察了小鼠大脑的轴突网络。将小鼠脑样本冷冻干燥以重建组织成分的内在对比度，并进行X射线可视化。通过吸收对比度可视化的整个大脑半球显示了包括纹状体、胼胝体和前连合的三维结构。在纹状体中观察到的轴突束起始于大脑皮质的基底表面，并终止于基底神经节中的各个位置。X射线衰减系数的分布表明，髓鞘和周围组织成分之间的水和磷脂含量的差异占所观察到的对比度。还用相位恢复方法分析了脑组织的杆状切口，其中组织微观结构可以以高达2.7 μm的分辨率分辨。通过追踪轴突束重建了纹状体的轴突网络结构。这样的分析应该能够描绘出所观察到的网络中所涉及的大脑区域的功能关系。
Neurons transmit active potentials through axons, which are essential for the brain to function. In this study, the axonal networks of the murine brain were visualized with X-ray tomographic microscopy, also known as X-ray microtomography or micro-CT. Murine brain samples were freeze-dried to reconstitute the intrinsic contrast of tissue constituents and subjected to X-ray visualization. A whole brain hemisphere visualized by absorption contrast illustrated three-dimensional structures including those of the striatum, corpus callosum, and anterior commissure. Axonal tracts observed in the striatum start from the basal surface of the cerebral cortex and end at various positions in the basal ganglia. The distribution of X-ray attenuation coefficients indicated that differences in water and phospholipid content between the myelin sheath and surrounding tissue constituents account for the observed contrast. A rod-shaped cutout of brain tissue was also analyzed with a phase retrieval method, wherein tissue microstructures could be resolved with up to 2.7 μm resolution. Structures of axonal networks of the striatum were reconstructed by tracing axonal tracts. Such an analysis should be able to delineate the functional relationships of the brain regions involved in the observed network.
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影响因子：
3.5
作者：
MANZOLI, FA;STEFONI, S;BARBIERI, M
通讯作者：
BARBIERI, M
影响因子：
48
作者：
Mikula, Shawn;Denk, Winfried
通讯作者：
Denk, Winfried
影响因子：
4.8
作者：
Mori S;Oishi K;Faria AV
通讯作者：
Faria AV
影响因子：
2
作者：
Paganin, D;Mayo, SC;Wilkins, SW
通讯作者：
Wilkins, SW
DOI：
10.1016/j.nima.2010.03.111
发表时间：
2010-09-01
影响因子：
1.4
作者：
Mizutani, Ryuta;Taguchi, Keisuke;Suzuki, Yoshio
通讯作者：
Suzuki, Yoshio