Disturbance by soil mixing decreases microbial richness and supports homogenizing community assembly processes.
Disturbance by soil mixing decreases microbial richness and supports homogenizing community assembly processes.
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DOI：
10.1093/femsec/fiac089
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发表时间：
2022-08-23
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
作者：
土壤微生境的空间异质性为在破坏土壤微生境和微生物群落固有空间隔离的物理干扰背景下研究生态模式和群落组装过程提供了依据。通过在16周的实验室孵育中以不同频率混合土壤，我们探索了物理干扰对土壤细菌丰富度、群落组成和群落组装过程的影响。我们假设，混合良好的土壤会孕育较少丰富的微生物群落，其群落组合以均匀分散和均匀选择为标志。利用16S rRNA基因测序，我们推断了群落的组装过程，估计了丰富度和差异丰度，并计算了组成不相似性。研究结果支持了我们的假设，在混合良好的土壤中，土壤细菌丰富度降低了约20%。土壤混合导致群落偏离未混合对照（Bray-Curtis dissimilarity; 0.75 vs. 0.25），同时降低了群内异质性。我们的研究结果表明，土壤中微生物群落的空间异质性和隔离性可能支持了土壤中微生物群落的巨大多样性，并为物理干扰和群落合并事件的影响提供了见解。通过分离和更好地理解空间异质性和不连通性对土壤微生物群落的影响，我们可以更好地推断人为干扰如何影响广泛的土壤功能。虽然土壤物理干扰（混合）导致细菌丰富度下降，土壤群落越来越相似，并且有证据表明群落组装过程均质化，但我们的研究结果还表明，由于土壤微环境的异质性和不连通性，土壤多样性在更大尺度上得以维持。
The spatial heterogeneity of soil’s microhabitats warrants the study of ecological patterns and community assembly processes in the context of physical disturbance that disrupts the inherent spatial isolation of soil microhabitats and microbial communities. By mixing soil at various frequencies in a 16-week lab incubation, we explored the effects of physical disturbance on soil bacterial richness, community composition, and community assembly processes. We hypothesized that well-mixed soil would harbor a less rich microbial community, with community assembly marked by homogenizing dispersal and homogeneous selection. Using 16S rRNA gene sequencing, we inferred community assembly processes, estimated richness and differential abundance, and calculated compositional dissimilarity. Findings supported our hypotheses, with > 20% decrease in soil bacterial richness in well-mixed soil. Soil mixing caused communities to diverge from unmixed controls (Bray–Curtis dissimilarity; 0.75 vs. 0.25), while reducing within-group heterogeneity. Our results imply that the vast diversity observed in soil may be supported by spatial heterogeneity and isolation of microbial communities, and also provide insight into the effects of physical disturbance and community coalescence events. By isolating and better understanding the effects of spatial heterogeneity and disconnectivity on soil microbial communities, we can better extrapolate how anthropogenic disturbances may affect broad soil functions. Though physical soil disturbance (mixing) results in decreased bacterial richness, increasingly similar soil communities, and evidence for homogenizing community assembly processes, our results also suggest that soil diversity is maintained at larger scales due to the heterogeneity and disconnectivity of soil microenvironments.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.9
作者：
Fine, Paul V. A.;Kembel, Steven W.
通讯作者：
Kembel, Steven W.
影响因子：
2.1
作者：
BOUYOUCOS, GJ
通讯作者：
BOUYOUCOS, GJ
影响因子：
--
作者：
BRAY, RH;KURTZ, LT
通讯作者：
KURTZ, LT
影响因子：
8.8
作者：
Adler, Peter B.;HilleRisLambers, Janneke;Levine, Jonathan M.
通讯作者：
Levine, Jonathan M.