Establishment, immortalisation and characterisation of pteropid bat cell lines.
Establishment, immortalisation and characterisation of pteropid bat cell lines.
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DOI：
10.1371/journal.pone.0008266
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发表时间：
2009-12-11
期刊：
影响因子：
3.7
通讯作者：
Wang LF
中科院分区：
文献类型：
--
作者：
Crameri G;Todd S;Grimley S;McEachern JA;Marsh GA;Smith C;Tachedjian M;De Jong C;Virtue ER;Yu M;Bulach D;Liu JP;Michalski WP;Middleton D;Field HE;Wang LF
蝙蝠被怀疑是包括尼帕病毒、亨德拉病毒、严重急性呼吸综合征冠状病毒和埃博拉病毒在内的许多新型和新出现的人畜共患病毒的天然宿主。自从在中国马蹄蝠中发现sars样冠状病毒以来，从蝙蝠中分离SL-CoV的尝试失败了，在各种哺乳动物细胞系中分离其他蝙蝠传播病毒的尝试也同样失败了。需要新的稳定的蝙蝠细胞系来帮助这些研究，并作为辅助研究蝙蝠免疫学和病毒-宿主相互作用的工具。从野外捕获黑狐（Pteropus alecto），并将其活体运送到实验室，使用各种不同的方法和培养基进行原代细胞培养制备。从20个不同器官中成功培养了原代细胞。细胞不朽可以自发发生，然而，我们使用逆转录病毒系统通过转移和稳定生产猿猴病毒40大T抗原和人类端粒酶逆转录酶蛋白来实现细胞不朽。使用亨德拉病毒和尼帕病毒对克隆和非克隆细胞系进行的初步感染实验表明，不同细胞系之间的感染效率不同程度，尽管有可能感染所有组织类型的细胞。本研究开发和优化的方法应该适用于其他物种的蝙蝠。我们正在使用本研究中建立的方法从许多不同的蝙蝠物种中产生进一步的细胞系。
Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions. Black flying foxes (Pteropus alecto) were captured from the wild and transported live to the laboratory for primary cell culture preparation using a variety of different methods and culture media. Primary cells were successfully cultured from 20 different organs. Cell immortalisation can occur spontaneously, however we used a retroviral system to immortalise cells via the transfer and stable production of the Simian virus 40 Large T antigen and the human telomerase reverse transcriptase protein. Initial infection experiments with both cloned and uncloned cell lines using Hendra and Nipah viruses demonstrated varying degrees of infection efficiency between the different cell lines, although it was possible to infect cells in all tissue types. The approaches developed and optimised in this study should be applicable to bats of other species. We are in the process of generating further cell lines from a number of different bat species using the methodology established in this study.
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影响因子：
11.8
作者：
Murray, Keith;Rogers, Russell;Selvey, Linda;Selleck, Paul;Hyatt, Alex;Gould, Allan;Gleeson, Laurie;Hooper, Peter;Westbury, Harvey
通讯作者：
Westbury, Harvey
影响因子：
3
作者：
Techangamsuwan, Somporn;Kreutzer, Robert;Baumgaertner, Wolfgang
通讯作者：
Baumgaertner, Wolfgang
DOI：
10.1179/096805104225005896
发表时间：
2004-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Takeuchi, O;Hemmi, H;Akira, S
通讯作者：
Akira, S
影响因子：
0.8
作者：
Middleton, D. J.;Morrissy, C. J.;Daniels, P. W.
通讯作者：
Daniels, P. W.
影响因子：
3.5
作者：
Meyerson, M
通讯作者：
Meyerson, M