A PPARγ, NF-κB and AMPK-dependent mechanism may be involved in the beneficial effects of curcumin in the diabetic db/db mice liver.
A PPARγ, NF-κB and AMPK-dependent mechanism may be involved in the beneficial effects of curcumin in the diabetic db/db mice liver.
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DOI：
10.3390/molecules19068289
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发表时间：
2014-06-18
期刊：
影响因子：
--
通讯作者：
Pérez-Vázquez V
中科院分区：
文献类型：
--
作者：
Jiménez-Flores LM;López-Briones S;Macías-Cervantes MH;Ramírez-Emiliano J;Pérez-Vázquez V
姜黄（Curcuma longa）是生姜科的一种多年生根茎草本植物，在阿育吠陀和中医中都被用来治疗胆道疾病、厌食症、咳嗽、风湿病、癌症、鼻窦炎、肝脏疾病、高血糖、肥胖和糖尿病。建议的作用机制包括信号转导级联的调节和对基因表达的影响，但它们仍有待阐明。本研究采用western blot方法，对15周龄db/db小鼠肝脏中姜黄素0.75%混合处理8周后的转录因子、炎症和代谢控制相关蛋白的表达进行了研究。此外，还对亚硝化胁迫进行了评价。姜黄素增加了db/db小鼠AMPK和PPARγ的表达，降低了NF-κB蛋白的表达。然而，它没有改变PGC-1α或SIRT1的表达。db/db小鼠肝脏中存在的亚硝化应激是由一个独特的硝基化蛋白带（75 kDa）决定的，姜黄素不会逆转亚硝化应激。综上所述，姜黄素调节AMPK、PPARγ、NF-κB的表达；提示对治疗T2DM并发症有有益作用。为了观察到最好的有益效果，在T2DM的早期状态给予姜黄素是可取的。
Turmeric (Curcuma longa) is a rhizomatous herbaceous perennial plant of the ginger family which has been used to treat biliary disorders, anorexia, cough, rheumatism, cancer, sinusitis, hepatic disorders, hyperglycemia, obesity, and diabetes in both Ayurvedic and Traditional Chinese Medicine. Suggested mechanisms of action include the modulation of signal transduction cascades and effects on gene expression, however they remain to be elucidated. In this study, the expression of some proteins responsible for transcription factors, inflammation, and metabolic control were evaluated by western blot in 15-week-old db/db mice livers treated with curcumin 0.75% mixed in their diet for 8 weeks. In addition, nitrosative stress was evaluated. Curcumin increased the expression of AMPK and PPARγ, and diminished NF-κB protein in db/db mice. However, it did not modify the expression of PGC-1α or SIRT1. Nitrosative stress present in db/db mice livers was determined by a unique nitrotyrosylated protein band (75 kDa) and was not reverted with curcumin. In conclusion, curcumin regulates the expression of AMPK, PPARγ, and NF-κB; suggesting a beneficial effect for treatment of T2DM complications. In order to observe best beneficial effects it is desirable to administer curcumin in the earlier states of T2DM.
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影响因子：
82.9
作者：
Arkan, MC;Hevener, AL;Karin, M
通讯作者：
Karin, M
影响因子：
4.1
作者：
Froejdoe, Sara;Durand, Christine;Pirola, Luciano
通讯作者：
Pirola, Luciano
影响因子：
7.7
作者：
Gillum MP;Kotas ME;Erion DM;Kursawe R;Chatterjee P;Nead KT;Muise ES;Hsiao JJ;Frederick DW;Yonemitsu S;Banks AS;Qiang L;Bhanot S;Olefsky JM;Sears DD;Caprio S;Shulman GI
通讯作者：
Shulman GI
影响因子：
4.8
作者：
Lan, Fan;Cacicedo, Jose M.;Ido, Yasuo
通讯作者：
Ido, Yasuo
DOI：
10.1139/apnm-2013-0133
发表时间：
2014-02-01
期刊：
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM-PHYSIOLOGIE APPLIQUEE NUTRITION ET METABOLISME
影响因子：
--
作者：
Franco-Robles, Elena;Campos-Cervantes, Alejandra;Ramirez-Emiliano, Joel
通讯作者：
Ramirez-Emiliano, Joel