Aerobic training lasting for 10 weeks elevates the adipose tissue FABP4, Giα, and adiponectin expression associated by a reduced protein oxidation.
Aerobic training lasting for 10 weeks elevates the adipose tissue FABP4, Giα, and adiponectin expression associated by a reduced protein oxidation.
复制标题
持续 10 周的有氧训练可提高脂肪组织 FABP4、Giα 和脂联素的表达，从而减少蛋白质氧化。
DOI：
10.4149/endo_2012_03_137
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发表时间：
2012
影响因子：
--
通讯作者：
Š. Zórad
中科院分区：
文献类型：
--
作者：
K. Krskova;M. Eckertova;M. Kukan;D. Kuba;A. Kebis;R. Olszanecki;M. Suski;L. Gajdošechová;Š. Zórad
目的
慢性适度运动能积极改变全身葡萄糖稳态，增强胰岛素作用，改善骨骼肌和肝脏的氧化损伤。本研究的目的是探讨间歇性有氧训练对肥胖Zucker大鼠白色脂肪组织代谢参数的影响。
方法
使8周龄的肥胖Zucker大鼠在4通道跑步机上跑步（1小时/天，5次/周，最多20 m/min）10周，周末除外（训练的肥胖Zucker，TOZ），或将其置于关闭的跑步机（久坐的肥胖Zucker，SOZ）上相同的时间段。测定血清胰岛素、血糖和甘油三酯。通过实时qPCR定量肝脏、附睾和腹膜后脂肪组织中的肾素-血管紧张素系统（RAS）组分和选定代谢参数的基因表达。测定肝脏和附睾脂肪贮库中蛋白质羰基的含量。
结果
脂肪细胞脂肪酸结合蛋白4（FABP 4）的基因表达在TOZ大鼠的附睾和腹膜后脂肪组织中显著升高。运动后腹膜后脂肪组织脂联素mRNA水平升高，附睾脂肪组织瘦素和抑制性G蛋白α mRNA水平升高。有氧训练导致附睾脂肪库中蛋白质羰基的数量减少。附睾脂肪组织中血管紧张素原、血管紧张素转换酶（ACE）和AT 1受体基因的转录不受运动的影响。在肝脏中，只有AT 1受体基因表达显著增加。与SOZ动物相比，TOZ大鼠的血清葡萄糖、胰岛素和甘油三酯浓度未发生变化。
结论
本研究的数据表明，在hyperphagic肥胖Zucker大鼠持续10周的间歇性适度运动改善了白色脂肪组织的一些形态和代谢参数，并降低了蛋白质氧化，这意味着长期运动的总体有益效果。
OBJECTIVE
The chronic moderate exercise positively alters the systemic glucose homeostasis, enhances the insulin action, and ameliorates the oxidative damage in the skeletal muscle and liver. The aim of this study was to investigate the effect of an intermittent aerobic training on the metabolic parameters of the white adipose tissue in the obese Zucker rats.
METHODS
Obese Zucker rats, 8 week old, were subjected to running on a 4-channel treadmill (1 h/day 5 times/week 20 m/min at maximum) for 10 weeks, except the weekends, (Trained Obese Zucker, TOZ) or were placed to the turned-off treadmill (Sedentary Obese Zucker, SOZ) for the same period. The serum insulin, glucose, and triglyceride were determined. The gene expression of the renin-angiotensin system (RAS) components and selected metabolic parameters were quantified by real-time qPCR in the liver and epididymal and retroperitoneal adipose tissues. The content of the protein carbonyl groups was assayed in the liver and epididymal fat depot.
RESULTS
The gene expression of the adipocyte fatty acid binding protein 4 (FABP4) was significantly elevated in the epididymal and retroperitoneal adipose tissues of the TOZ rats. The level of the adiponectin mRNA was increased in the retroperitoneal adipose tissue while leptin and inhibitory G-protein α mRNA were elevated in the epididymal adipose tissue after exercise. The aerobic training led to a decrease in the amount of protein carbonyl groups in the epididymal adipose depot. Transcription of the angiotensinogen, angiotensin-converting enzyme (ACE), and AT1 receptor genes in the epididymal adipose tissue was not influenced by the exercise. In the liver, only the AT1 receptor gene expression increased significantly. The serum glucose, insulin, and triglycerides concentrations were not changed in the TOZ rats when compared to SOZ animals.
CONCLUSIONS
Data of the present study indicate that an intermittent moderate exercise in the hyperphagic obese Zucker rats lasting for 10 weeks improves some of the morphometric and metabolic parameters of the white adipose tissue and decreases the protein oxidation implying a general beneficial effect of the long-lasting exercising.
荧光脂肪酸从脂肪细胞脂肪酸结合蛋白转移到细胞膜的机制。
影响因子：
2.9
作者：
Wootan,MG;Bernlohr,DA;Storch,J
通讯作者：
Storch,J