Mechanism of fluorescent fatty acid transfer from adipocyte fatty acid binding protein to membranes.
Mechanism of fluorescent fatty acid transfer from adipocyte fatty acid binding protein to membranes.
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荧光脂肪酸从脂肪细胞脂肪酸结合蛋白转移到细胞膜的机制。
DOI：
10.1021/bi00084a033
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Storch,J
中科院分区：
文献类型：
--
作者：
Wootan,MG;Bernlohr,DA;Storch,J
修订稿于 1993 年 5 月 4 日收到 摘要：脂肪细胞脂肪酸结合蛋白 (A-FABP) 是一种在脂肪细胞胞浆中大量存在的 15 kDa 蛋白质。为了更好地了解该蛋白质在细胞内游离脂肪酸 (ffa) 转运中的作用，通过使用共振能量转移测定法监测荧光蒽酰氧基 ffa (AOffa) 向小单层磷脂囊泡的转移，研究了 FFA 从 A-FABP 转移到模型膜的机制。 FFA 增加水溶性的结构特征，例如较短的链长和不饱和度，并没有增加 AOffa 转移速率。此外，增加FFA水溶性的溶液条件，例如降低离子强度和增加pH值，对AOffa从A-FABP到膜的转移几乎没有影响。这些结果表明 AOffa 不通过水相转移。对转移过程自由能的小熵贡献进一步证明，当从 A-FABP 转移到磷脂膜时，AOffa 可能不会穿过周围的水环境。最后，从 A-FABP 转移 AOffa 的速率直接取决于受体膜的浓度。这些研究表明，AOffa 从 A-FABP 到磷脂囊泡的转移可能是通过蛋白质和膜之间的瞬时碰撞相互作用发生的。这种机制类似于最近发现的从心脏 FABP 转移 AOffa 的机制 [Kim,. K., & Storch, J.(1992) J. Biol。化学。 267, 20051-20056]，一种与 A-FABP 具有高度序列同源性（62% 同一性）的 FABP，但与更远相关（20% 同源性）肝脏 FABP 所描述的水扩散机制不同 [Kim,. K., & Storch, J.(1992) J. Biol。化学。 267、77-82]。这些差异表明 FABP 之间的结构差异可能会转化为功能差异，正如 AOffa 转移到膜的机制所证明的那样。
Revised Manuscript Received May 4, 1993 abstract: Adipocyte fatty acid binding protein (A-FABP) is a 15-kDa protein found in high abundance in the cytosol of adipose cells. Tobetter understand the role of this protein in intracellular free fatty acid (ffa) transport, the mechanism of ffa transfer from A-FABP to model membranes was examined by monitoring the transfer of fluorescent anthroyloxy ffa (AOffa) to smallunilamellar phospholipid vesicles, using a resonance energy transfer assay. Structural features of ffa that increase aqueous solubility, such as shorter chain length and unsaturation, did not increase the AOffa transfer rate. In addition, solution conditions that increase the aqueous solubility of ffa, such as decreasing ionic strength and increasing pH, had little effect on AOffa transfer from A-FABP to membranes. These results suggest that AOffa do not transfer through theaqueous phase. The smallentropic contribution to the free energy of the transfer process provides further evidence that AOffa maynot travel through the surrounding aqueous environment when transferred from A-FABP to phospholipid membranes. Finally, the rate of AOffa transfer from A-FABP was directly dependent on the concentration of the acceptor membranes. These studies suggest that AOffa transfer from A-FABP to phospholipid vesicles may occur via transient collisional interactions between the protein and membranes. Such a mechanism is similar to that found recently for AOffa transfer from heart FABP [Kim,. K., & Storch, J.(1992) J. Biol. Chem. 267, 20051-20056], an FABP which possesses a high degree of sequence homology (62% identity) with A-FABP, but different from the aqueous diffusion mechanism described for the more distantly related (20% homology) liverFABP [Kim,. K., & Storch, J.(1992) J. Biol. Chem. 267, 77-82]. These differences indicate that structural divergence among FABP may be translated into functional differences, as evidenced here by the mechanism of AOffa transfer to membranes.
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双层膜之间磷脂交换的动力学。
DOI：
10.1016/0005-2736(77)90168-7
发表时间：
1977
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
L. Thilo
通讯作者：
L. Thilo
牛周围神经髓磷脂碱性蛋白的分布和光学活性
影响因子：
4.7
作者：
K. Uyemura;T. Kato;K. Kitamura
通讯作者：
K. Kitamura
将荧光脂肪酸从肝脏和心脏脂肪酸结合蛋白转移到模型膜。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Storch,J;Bass,NM
通讯作者：
Bass,NM
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Steck,TL;Kezdy,FJ;Lange,Y
通讯作者：
Lange,Y
DOI：
10.1073/pnas.81.17.5468
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BERNLOHR, DA;ANGUS, CW;KELLY, TJ
通讯作者：
KELLY, TJ