Evaluation of variation within the barcode region of Cytochrome c Oxidase I (COI) for the detection of commercial Callinectes sapidus Rathbun, 1896 (blue crab) products of non-US origin.
Evaluation of variation within the barcode region of Cytochrome c Oxidase I (COI) for the detection of commercial Callinectes sapidus Rathbun, 1896 (blue crab) products of non-US origin.
复制标题
评估细胞色素 c 氧化酶 I (COI) 条形码区域内的变异，用于检测非美国原产的商业 Callinectes sapidus Rathbun, 1896（蓝蟹）产品。
作者：
A. Windsor;M. Moore;Kimberly A. Warner;Sarah R. Stadig;Jonathan Deeds
Callinectes sapidus Rathbun, 1896 是一种西大西洋物种，其自然地理分布范围从美国马萨诸塞州到委内瑞拉（分布区 1）以及从巴西阿拉戈斯州到阿根廷北部（分布区 2）。它是在美国大陆商业捕捞的唯一梭子蟹品种，但也从几个拉丁美洲国家（特别是委内瑞拉和墨西哥）进口到美国。在美国，标有“蓝蟹”和“美国产品”的蟹产品可能不合法地含有非美国捕捞的其他蟹类或 C. sapidus 品种。本研究记录了从整个自然范围收集的 417 个 C. sapidus 参考样本中细胞色素 c 氧化酶 I (COI) 条形码区域内的核苷酸变异。本研究的目的是通过将参考样本中的基因特征与标记为“美国产品”和“委内瑞拉产品”的商业蟹肉中观察到的基因特征进行比较，确定是否可以利用这种变异来检测在州际贸易中销售的贴错标签的 C. sapidus 产品。在参考样本中，我们观察到条形码区域存在高水平的遗传变异。然而，一致地观察到三个谱系，谱系之间具有显着的成对 F st 值。谱系 1 在整个自然地理范围内都有观察到，但在美国大陆占主导地位，并且是在主要蟹肉生产州（马里兰州、洛杉矶、弗吉尼亚州、北卡罗来纳州）观察到的唯一谱系。谱系2主要出现在分布区1的加勒比地区，但在美国海岸的南大西洋湾地区也很少遇到。最后，谱系 3 仅在巴西水域中观察到，并且具有最低的单倍型和核苷酸多样性值。谱系 1 和 2 的平均配对距离（p 距离）为 3.15%，而谱系 3 与谱系 1 和 2 的平均 p 距离分别为 2.55% 和 1.35%。谱系内，谱系 1、2 和 3 的平均 p 距离分别为 0.45%、0.19% 和 0.07%。在从美国大陆、墨西哥、波多黎各和委内瑞拉收集的所有有凭证的参考标本中，我们确定了 22 个系统发育信息丰富的位点，这些位点驱动了观察到的谱系分歧。从标记为源自美国的商业 C. sapidus 产品的条形码 COI 序列中鉴定出的单倍型均与来自谱系 1 参考样本的单倍型以及来自标记为源自委内瑞拉的商业产品的单倍型均与谱系 2 对齐，这表明这些谱系可能有助于在包装标签有问题时指示产品是否源自美国大陆或进口。
Callinectes sapidus Rathbun, 1896 is a western Atlantic species with a disjointed natural geographic range from Massachusetts, USA to Venezuela (distribution area 1) and from Alagoas, Brazil to northern Argentina (distribution area 2). It is the only species of portunid crab commercially harvested in the continental United States but is also imported into the US from several Latin American countries, Venezuela and Mexico in particular. In the United States, crab products labeled as "blue crab" and "Product of the USA" may not legally contain other species of crab or C. sapidus not harvested in the United States. The present study documents nucleotide variation within the barcode region of cytochrome c oxidase I (COI) in 417 reference specimens of C. sapidus collected from throughout its natural range. The goal of this study is to determine if this variation can be utilized to detect mislabeled C. sapidus products sold in interstate commerce by comparing genetic signatures in reference specimens to those observed in commercial crabmeat labeled as "Product of the USA" and "Product of Venezuela." In reference specimens, we observed high levels of genetic variation in the barcode region. However, three lineages were consistently observed with significant pairwise F st values between the lineages. Lineage 1 was observed throughout the natural geographic range but predominated in the continental US and was the only lineage observed in the major crabmeat-producing states (MD, LA, VA, NC). Lineage 2 primarily occurred in the Caribbean region of distribution area 1 but was also infrequently encountered in the South Atlantic Bight region of the US coast. Finally, Lineage 3 was only observed in Brazilian waters and had the lowest haplotype and nucleotide diversity values. Lineages 1 and 2 were separated by a mean pairwise distance (p-distance) of 3.15%, whereas Lineage 3 had a mean p-distance of 2.55% and 1.35% to Lineages 1 and 2, respectively. Within lineage mean p-distances were 0.45%, 0.19%, and 0.07% for Lineages 1, 2, and 3, respectively. Among all vouchered reference specimens collected from the continental United States, Mexico, Puerto Rico, and Venezuela, we identified 22 phylogenetically informative sites that drive observed lineage divergences. Haplotypes identified from barcode COI sequences from commercial C. sapidus products labeled as originating from the US all aligned with haplotypes from Lineage 1 reference specimens and haplotypes from commercial products labeled as originating from Venezuela all aligned with Lineage 2, suggesting that these lineages may be useful for indicating whether products originate from the continental US or are imported when package labeling is in question.
影响因子：
2.7
作者：
Deng W;Maust BS;Nickle DC;Learn GH;Liu Y;Heath L;Kosakovsky Pond SL;Mullins JI
通讯作者：
Mullins JI
影响因子：
2.7
作者：
Dudoit ';Iacchei M;Coleman RR;Gaither MR;Browne WE;Bowen BW;Toonen RJ
通讯作者：
Toonen RJ