A Rad26–Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
A Rad26–Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
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Rad26-Def1 复合物协调修复和 RNA pol II 蛋白水解以响应 DNA 损伤
作者：
Elies C. Woudstra;C. Gilbert;J. Fellows;L. Jansen;J. Brouwer;H. Erdjument;P. Tempst;J. Svejstrup
Eukaryotic cells use multiple, highly conserved mechanisms to contend with ultraviolet-light-induced DNA damage. One important response mechanism is transcription-coupled repair (TCR), during which DNA lesions in the transcribed strand of an active gene are repaired much faster than in the genome overall. In mammalian cells, defective TCR gives rise to the severe human disorder Cockayne's syndrome (CS). The best-studied CS gene, CSB, codes for a Swi/Snf-like DNA-dependent ATPase, whose yeast homologue is called Rad26 (ref. 4). Here we identify a yeast protein, termed Def1, which forms a complex with Rad26 in chromatin. The phenotypes of cells lacking DEF1 are consistent with a role for this factor in the DNA damage response, but Def1 is not required for TCR. Rather, def1 cells are compromised for transcript elongation, and are unable to degrade RNA polymerase II (RNAPII) in response to DNA damage. Our data suggest that RNAPII stalled at a DNA lesion triggers a coordinated rescue mechanism that requires the Rad26–Def1 complex, and that Def1 enables ubiquitination and proteolysis of RNAPII when the lesion cannot be rapidly removed by Rad26-promoted DNA repair.
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DOI：
10.1016/s0921-8777(01)00097-0
发表时间：
2001-09-04
期刊：
MUTATION RESEARCH-DNA REPAIR
影响因子：
--
作者：
Luo, ZH;Zheng, JH;Bregman, DB
通讯作者：
Bregman, DB
使用多元醇响应单克隆抗体对 RNA 聚合酶 II 和转录因子进行免疫亲和纯化。
影响因子：
--
作者：
Thompson,NE;Burgess,RR
通讯作者：
Burgess,RR
DOI：
10.1016/s0921-8777(00)00071-9
发表时间：
2001-02-25
期刊：
MUTATION RESEARCH-DNA REPAIR
影响因子：
--
作者：
Hanawalt, PC
通讯作者：
Hanawalt, PC
DOI：
10.1073/pnas.83.14.5189
发表时间：
1986-07-01
影响因子：
11.1
作者：
FIELDS, PI;SWANSON, RV;HEFFRON, F
通讯作者：
HEFFRON, F
DOI：
10.1016/s0921-8777(00)00064-1
发表时间：
2001-03-07
期刊：
MUTATION RESEARCH-DNA REPAIR
影响因子：
--
作者：
McKay, BC;Chen, F;Ljungman, M
通讯作者：
Ljungman, M