Producing aglycons of ginsenosides in bakers' yeast.
Producing aglycons of ginsenosides in bakers' yeast.
复制标题
在面包酵母中生产人参皂苷苷元
DOI：
10.1038/srep03698
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发表时间：
2014-01-15
影响因子：
4.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Dai Z;Wang B;Liu Y;Shi M;Wang D;Zhang X;Liu T;Huang L;Zhang X
人参皂苷是人参的主要生物活性成分，人参是一种流行的药用植物，具有多种药理活性。原人参二醇、原人参三醇和齐墩果酸是人参皂苷的三种基本苷元。本工作实现了在酿酒酵母中生产人参皂苷苷元，与传统提取方法相比，提供了一条替代途径。这三个苷元的合成途径是在S中构建的。通过引入来自不同植物的β-香树脂醇合酶、齐墩果酸合酶、达玛烯二醇-II合酶、原人参二醇合酶、原人参三醇合酶和NADPH-细胞色素P450还原酶。此外，截短的3-羟基-3-甲基戊二酰辅酶A还原酶、角鲨烯合酶和2,3-氧化角鲨烯合酶基因被过表达，以增加前体供应以改善苷元生产。获得菌株GY-1，其产原人参二醇17.2mg/L，原人参三醇15.9mg/L，齐墩果酸21.4mg/L。这项工作中工程化的酵母菌株可以作为创建替代植物来源提取物生产人参皂苷的替代方法的基础。
Ginsenosides are the primary bioactive components of ginseng, which is a popular medicinal plant that exhibits diverse pharmacological activities. Protopanaxadiol, protopanaxatriol and oleanolic acid are three basic aglycons of ginsenosides. Producing aglycons of ginsenosides inSaccharomyces cerevisiaewas realized in this work and provides an alternative route compared to traditional extraction methods. Synthetic pathways of these three aglycons were constructed inS. cerevisiaeby introducing β-amyrin synthase, oleanolic acid synthase, dammarenediol-II synthase, protopanaxadiol synthase, protopanaxatriol synthase and NADPH-cytochrome P450 reductase from different plants. In addition, a truncated 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthase and 2,3-oxidosqualene synthase genes were overexpressed to increase the precursor supply for improving aglycon production. Strain GY-1 was obtained, which produced 17.2 mg/L protopanaxadiol, 15.9 mg/L protopanaxatriol and 21.4 mg/L oleanolic acid. The yeast strains engineered in this work can serve as the basis for creating an alternative way for producing ginsenosides in place of extractions from plant sources.
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影响因子：
3.8
作者：
Qi LW;Wang CZ;Yuan CS
通讯作者：
Yuan CS
影响因子：
8.4
作者：
Ma, Suzanne M.;Garcia, David E.;Chhabra, Swapnil R.
通讯作者：
Chhabra, Swapnil R.
影响因子：
3.8
作者：
Dai, Zhubo;Liu, Yi;Zhang, Xueli
通讯作者：
Zhang, Xueli
DOI：
10.1016/s0167-4781(99)00035-4
发表时间：
1999-04-14
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Kennedy, MA;Barbuch, R;Bard, M
通讯作者：
Bard, M
影响因子：
64.8
作者：
Ro, DK;Paradise, EM;Keasling, JD
通讯作者：
Keasling, JD