Full picture of the thermotropic phase behavior of cardiolipin bilayer in water: identification of a metastable subgel phase
Full picture of the thermotropic phase behavior of cardiolipin bilayer in water: identification of a metastable subgel phase
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心磷脂双层在水中的热致相行为的全貌：亚稳态亚凝胶相的鉴定
DOI：
10.1039/c4ra09158b
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发表时间：
2014-10
期刊：
影响因子：
3.9
通讯作者：
Yu, Zhi-Wu
中科院分区：
文献类型：
--
作者：
Sun, Hai-Yuan;Zhou, Yu;Wu, Rui-Guang;Yu, Zhi-Wu
从流体相形成稳定的结晶相通常涉及在冷却期间通过凝胶或亚稳定的亚凝胶相。在此，我们首次发现，对于四尾脂质，四肉豆蔻酰心磷脂（TMCL），在从凝胶相冷却时形成亚稳亚凝胶相（“亚凝胶II”型相，或SGII），并且这样的亚稳亚凝胶相充当形成稳定结晶相的前体。进行了详细的热致性、X-射线衍射和红外光谱研究，以研究亚凝胶相和结晶相的形成，沿着比较凝胶相和流体相。所有四个相（结晶，subgel，凝胶和流体）是层状结构和凝胶和流体相的层间距溶胀的水含量增加在纯水中。得到了TMCL在水中的热致相行为的全貌，发现流体-凝胶和凝胶-亚凝胶之间的转变是可逆的，而涉及结晶相的相变过程是不可逆的。凝胶到亚凝胶的转变主要涉及尾部堆积状态的重组（从六方到正交），并且发现这种相变与溶剂无关。从亚凝胶到结晶相的转变需要在相对较低的温度（0-5 °C）下长的孵育时间，并且该结晶相在加热时转化为凝胶相。本工作揭示了两亲分子的结晶过程，并对心磷脂模型体系或超分子自组装体系的稳定性具有深远的意义。
The formation of a stable crystalline phase from a fluid phase usually involves the passage through a gel or metastable subgel phase during cooling. Herein, we found for the first time that for the four-tailed lipid, tetramyristoyl cardiolipin (TMCL), a metastable subgel phase (“subgel II”-type phase, or SGII) was formed upon cooling from the gel phase, and such a metastable subgel phase served as the precursor towards the formation of a stable crystalline phase. A detailed thermotropic, X-ray diffraction and infrared spectroscopic study was carried out to study the formation of the subgel and crystalline phases, along with a comparison of the gel and fluid phases. All the four phases (crystalline, subgel, gel and fluid) are lamellar structured and the interlamellar spacings of the gel and fluid phases swell as the water content increases in pure water. A full picture of the thermotropic phase behavior of TMCL in water was obtained and it was found that the transformations between fluid–gel and gel–subgel are reversible, while the phase transformation processes involving the crystalline phase are irreversible. The gel to subgel transition mainly involves the reorganization of the tail packing state (from hexagonal to orthorhombic), and this phase transition was found to be independent of solvent. Changing from subgel to crystalline phase needs a long incubation time at relatively low temperatures (0–5 °C), and this crystalline phase converts to a gel phase upon heating. The present work sheds light on the crystallization process of amphiphiles and has profound implications on the stability of cardiolipin-based model systems or supramolecular self-assembly systems.
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影响因子：
3.9
作者：
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