The putative pectin methylesterase gene, BcMF23a, is required for microspore development and pollen tube growth in Brassica campestris
The putative pectin methylesterase gene, BcMF23a, is required for microspore development and pollen tube growth in Brassica campestris
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假定的果胶甲酯酶基因 BcMF23a 是油菜小孢子发育和花粉管生长所必需的
DOI：
10.1007/s00299-018-2285-6
复制
发表时间：
2018-04
影响因子：
6.2
通讯作者：
曹家树
中科院分区：
文献类型：
--
作者：
岳晓艳;曹家树
花粉壁是花粉的外表面，它包裹着雄配子体，在植物生殖过程中起着重要作用。果胶甲基酯酶（PMEs）参与花粉壁的构建，通过将内壁的果胶脱乙酰化。本研究对油菜果胶甲酯酶基因BcMF23a的功能进行了研究。通过人工microRNA（amiRNA）技术敲除BcMF23a，导致双核期萌发沟外的花粉内壁异常形成。在三核期，20.69%的花粉核、细胞质和内壁降解，花粉皱缩，其余75.86%的花粉胞壁破裂，细胞质降解，外壁破裂，位于萌发沟内。此外，转基因植株的花粉败育导致离体萌发率降低19%，而在活体内则导致天然柱头中花粉管生长中断。综上所述，BcMF23 a可能通过参与内壁的构建而参与花粉发育和花粉管生长。本研究有助于进一步了解花粉特异性质膜微囊的功能和花粉壁发育的分子调控网络。
Pollen wall, the morphological out face of pollen, surrounds male gametophyte and plays an important role in plant reproduction. Pectin methylesterases (PMEs) are involved in pollen wall construction by de-esterifying pectin of the intine. In this study, the function of a putative pectin methylesterase gene, Brassica campestris Male Fertility 23a (BcMF23a), was investigated. Knockdown of BcMF23a by artificial microRNA (amiRNA) technology resulted in abnormal pollen intine formation outside of the germinal furrows at the binucleate stage. At the trinucleate stage, 20.69% of pollen possessed the degradation of nuclei, cytoplasm and the intine, resulting in shrunken pollen, whereas the remaining 75.86% were wall-disrupted with degrading cytoplasm and broken exine inside the germinal furrows. In addition, pollen abortion in transgenic plants caused germination percentage reduction by 19% in vitro and pollen tube growth disruption in natural stigma in vivo. Taken together, BcMF23a is involved in pollen development and pollen tube growth, possibly via participating in intine construction. This study may contribute towards understanding the function of pollen-specific PMEs and the molecular regulatory network of pollen wall development.
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DOI：
10.1080/14620316.2004.11511851
发表时间：
2004-01
期刊：
The Journal of Horticultural Science and Biotechnology
影响因子：
--
作者：
Xiaolin Yu;Jiashu Cao;W. Ye;Yongqin Wang
通讯作者：
Xiaolin Yu;Jiashu Cao;W. Ye;Yongqin Wang
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
D. Honys;D. Rĕnák;D. Twell;J. D. Silva
通讯作者：
D. Honys;D. Rĕnák;D. Twell;J. D. Silva
影响因子：
7.4
作者：
Dardelle, Flavien;Lehner, Arnaud;Mollet, Jean-Claude
通讯作者：
Mollet, Jean-Claude
DOI：
10.3390/plants2010107
发表时间：
2013-03-07
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Mollet JC;Leroux C;Dardelle F;Lehner A
通讯作者：
Lehner A
影响因子：
18.2
作者：
Ramesh Katam;Dilip R. Panthee;E. Basenko;R. Bandopadhyay;Sheikh M. Basha;Kokiladevi Eswaran;
通讯作者：
Ramesh Katam;Dilip R. Panthee;E. Basenko;R. Bandopadhyay;Sheikh M. Basha;Kokiladevi Eswaran;