Isolation and characterization of arylacetamide deacetylase in cynomolgus macaques.
Isolation and characterization of arylacetamide deacetylase in cynomolgus macaques.
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DOI：
10.1292/jvms.14-0496
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Imai T
中科院分区：
文献类型：
--
作者：
Uno Y;Hosokawa M;Imai T
芳基乙酰胺去乙酰化酶（AADAC）是一种微粒体丝氨酸酯酶，可水解药物，如氟他胺、非那西丁和利福平。由于在药物代谢研究中广泛使用的非人类灵长类动物食蟹猴中，AADAC尚未在分子水平上得到充分的研究，因此我们对食蟹猴AADAC cDNA进行了分离和表征。推导出的氨基酸序列与人类AADAC高度同源（92%），在系统发育树中与人类AADAC的同源性比狗、大鼠或小鼠更接近。在食蟹和人类基因组中，AADAC被AADACL2和SUCNR1所包围。与人类相似，食蟹猕猴的肝脏和空肠等药物代谢器官中也有相对丰富的AADAC mRNA表达。结果表明食蟹猕猴与人类的AADAC分子具有相似性。
Arylacetamide deacetylase (AADAC), a microsomal serine esterase, hydrolyzes drugs, such as flutamide, phenacetin and rifampicin. Because AADAC has not been fully investigated at molecular levels in cynomolgus macaques, the non-human primate species widely used in drug metabolism studies, cynomolgus AADAC cDNA was isolated and characterized. The deduced amino acid sequence, highly homologous (92%) to human AADAC, was more closely clustered with human AADAC than the dog, rat or mouse ortholog in a phylogenetic tree. AADAC was flanked by AADACL2 and SUCNR1 in the cynomolgus and human genomes. Moreover, relatively abundant expression of AADAC mRNA was found in liver and jejunum, the drug-metabolizing organs, in cynomolgus macaques, similar to humans. The results suggest molecular similarities of AADAC between cynomolgus macaques and humans.
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