Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression.
Exploring the Role of Epicardial Adipose Tissue in Coronary Artery Disease From the Difference of Gene Expression.
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从基因表达差异探讨心外膜脂肪组织在冠心病中的作用
DOI：
10.3389/fphys.2021.605811
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发表时间：
2021
影响因子：
4
通讯作者：
Shi RZ
中科院分区：
文献类型：
--
作者：
Wang QC;Wang ZY;Xu Q;Li RB;Zhang GG;Shi RZ
目的心外膜脂肪组织（Epicardial adipose tissue，EAT）与冠状动脉和心肌组织密切相关，其作为内分泌器官影响冠状动脉和心肌组织的病理生理过程的作用日益受到重视。然而，冠状动脉疾病（CAD）中EAT的特异性基因表达谱尚未得到很好的表征。我们的目的是在基因水平上研究EAT在CAD中的作用。方法比较冠心病与非冠心病患者EAT的组织学和基因表达差异。我们通过高通量RNA测序技术研究了CAD患者EAT的基因表达谱。通过功能富集分析、蛋白质相互作用网络构建等生物信息学分析，获得并验证CAD EAT中的中枢差异表达基因（DEG）。结果冠心病组心外膜脂肪细胞体积大于对照组。我们对CAD的EAT基因表达谱的发现显示总共747个DEG（倍数变化>2，p值<0.05）。DEGs富集分析显示CAD中涉及更多的促炎和免疫基因及通路。确定了10个中心DEG（GNG 3、MCHR 1、BDKRB 1、MCHR 2、CXCL 8、CXCR 5、CCR 8、CCL 4L1、TAS2R10和TAS2R41）。结论CAD患者心外膜脂肪组织具有独特的基因表达谱，可能是CAD病理过程中的关键调控因子。
Objectives Epicardial adipose tissue (EAT) is closely adjacent to the coronary arteries and myocardium, its role as an endocrine organ to affect the pathophysiological processes of the coronary arteries and myocardium has been increasingly recognized. However, the specific gene expression profiles of EAT in coronary artery disease (CAD) has not been well characterized. Our aim was to investigate the role of EAT in CAD at the gene level. Methods Here, we compared the histological and gene expression difference of EAT between CAD and non-CAD. We investigated the gene expression profiles in the EAT of patients with CAD through the high-throughput RNA sequencing. We performed bioinformatics analysis such as functional enrichment analysis and protein-protein interaction network construction to obtain and verify the hub differentially expressed genes (DEGs) in the EAT of CAD. Results Our results showed that the size of epicardial adipocytes in the CAD group was larger than in the control group. Our findings on the EAT gene expression profiles of CAD showed a total of 747 DEGs (fold change >2, p value <0.05). The enrichment analysis of DEGs showed that more pro-inflammatory and immunological genes and pathways were involved in CAD. Ten hub DEGs (GNG3, MCHR1, BDKRB1, MCHR2, CXCL8, CXCR5, CCR8, CCL4L1, TAS2R10, and TAS2R41) were identified. Conclusion Epicardial adipose tissue in CAD shows unique gene expression profiles and may act as key regulators in the CAD pathological process.
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影响因子：
9.3
作者：
Bouchi R;Terashima M;Sasahara Y;Asakawa M;Fukuda T;Takeuchi T;Nakano Y;Murakami M;Minami I;Izumiyama H;Hashimoto K;Yoshimoto T;Ogawa Y
通讯作者：
Ogawa Y
影响因子：
8.3
作者：
Franssens, Bas T.;Nathoe, Hendrik M.;Visseren, Frank L. J.
通讯作者：
Visseren, Frank L. J.
影响因子：
10.8
作者：
Gast, Martina;Rauch, Bernhard H.;Poller, Wolfgang
通讯作者：
Poller, Wolfgang
影响因子：
1.6
作者：
McKenney ML;Schultz KA;Boyd JH;Byrd JP;Alloosh M;Teague SD;Arce-Esquivel AA;Fain JN;Laughlin MH;Sacks HS;Sturek M
通讯作者：
Sturek M
影响因子：
5.6
作者：
Ding, Ru;Gao, Wenwu;Liang, Chun
通讯作者：
Liang, Chun