Accessory Genome Dynamics of Local and Global Staphylococcus pseudintermedius Populations.
Accessory Genome Dynamics of Local and Global Staphylococcus pseudintermedius Populations.
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DOI：
10.3389/fmicb.2022.798175
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发表时间：
2022
影响因子：
5.2
通讯作者：
Andam CP
中科院分区：
文献类型：
--
作者：
Bruce SA;Smith JT;Mydosh JL;Ball J;Needle DB;Gibson R;Andam CP
假中间葡萄球菌是犬的主要定植菌和条件致病菌。耐甲氧西林表皮葡萄伪中间肌（MRSP）继续成为维持犬健康的重大挑战。我们试图确定系统发育关系的S。pseudintermedius横跨美国新英格兰地区的五个州，并将它们置于全球背景下。新英格兰的数据集包括125个以前发表的S. pseudintermedius基因组补充了45个新测序的分离株。核心基因组系统发育树揭示了由142个多位点序列类型（ST）组成的许多深分支谱系。mecA基因的计算机检测显示40例MRSP和130例甲氧西林敏感的S。假中间体（MSSP）分离株。MRSP来源于SCCmec的五种结构类型，SCCmec是携带赋予甲氧西林抗性的mecA基因的移动的遗传元件。虽然许多基因组是MSSP，但它们仍然含有赋予对许多其他抗生素类别的抗性的基因，包括氨基糖苷类、大环内酯类、四环素类和青霉烷类。我们将新英格兰的基因组与之前公布的来自美国其他五个州和其他13个国家的297个基因组进行了比较。尽管在世界范围内和美国其他地区发现了克隆扩展的ST 71的流行，但我们在新英格兰没有检测到它。接下来，我们试图询问合并的新英格兰和全球数据集是否存在与抗生素耐药性相关的重合基因对。分析显示了一个大的共循环辅助基因簇，其中包括mecA以及其他8个抗性基因[aac（6′）-Ie-aph（2″）-Ia，aad（6），aph（3′）-IIIa，sat 4，ermB，cat，blaZ和tetM]。此外，MRSP分离株携带的辅助基因明显多于MSSP。我们的研究结果为高风险克隆的进化和地理分布提供了重要的见解，这些克隆可能会威胁到我们犬同伴的健康。
Staphylococcus pseudintermedius is a major bacterial colonizer and opportunistic pathogen in dogs. Methicillin-resistant S. pseudintermedius (MRSP) continues to emerge as a significant challenge to maintaining canine health. We sought to determine the phylogenetic relationships of S. pseudintermedius across five states in the New England region of the United States and place them in a global context. The New England dataset consisted of 125 previously published S. pseudintermedius genomes supplemented with 45 newly sequenced isolates. The core genome phylogenetic tree revealed many deep branching lineages consisting of 142 multi-locus sequence types (STs). In silico detection of the mecA gene revealed 40 MRSP and 130 methicillin-susceptible S. pseudintermedius (MSSP) isolates. MRSP were derived from five structural types of SCCmec, the mobile genetic element that carries the mecA gene conferring methicillin resistance. Although many genomes were MSSP, they nevertheless harbored genes conferring resistance to many other antibiotic classes, including aminoglycosides, macrolides, tetracyclines and penams. We compared the New England genomes to 297 previously published genomes sampled from five other states in the United States and 13 other countries. Despite the prevalence of the clonally expanding ST71 found worldwide and in other parts of the United States, we did not detect it in New England. We next sought to interrogate the combined New England and global datasets for the presence of coincident gene pairs linked to antibiotic resistance. Analysis revealed a large co-circulating accessory gene cluster, which included mecA as well as eight other resistance genes [aac (6′)-Ie-aph (2″)-Ia, aad (6), aph (3′)-IIIa, sat4, ermB, cat, blaZ, and tetM]. Furthermore, MRSP isolates carried significantly more accessory genes than their MSSP counterparts. Our results provide important insights to the evolution and geographic spread of high-risk clones that can threaten the health of our canine companions.
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DOI：
10.1093/jac/dkt418
发表时间：
2014-03
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Ba X;Harrison EM;Edwards GF;Holden MT;Larsen AR;Petersen A;Skov RL;Peacock SJ;Parkhill J;Paterson GK;Holmes MA
通讯作者：
Holmes MA
影响因子：
2.6
作者：
Gagetti, Paula;Wattam, Alice R.;Rosato, Adriana E.
通讯作者：
Rosato, Adriana E.
DOI：
10.1093/bioinformatics/bty095
发表时间：
2018-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bertelli C;Brinkman FSL
通讯作者：
Brinkman FSL
影响因子：
9.4
作者：
Duim, Birgitta;Verstappen, Koen M.;Wagenaar, Jaap A.
通讯作者：
Wagenaar, Jaap A.
影响因子：
5.2
作者：
Couto, Natacha;Monchique, Claudia;Pomba, Constanca
通讯作者：
Pomba, Constanca