Small RNAs Gained during Epididymal Transit of Sperm Are Essential for Embryonic Development in Mice.
Small RNAs Gained during Epididymal Transit of Sperm Are Essential for Embryonic Development in Mice.
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DOI：
10.1016/j.devcel.2018.06.024
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发表时间：
2018-08-20
影响因子：
11.8
通讯作者：
Rando OJ
中科院分区：
文献类型：
--
作者：
Conine CC;Sun F;Song L;Rivera-Pérez JA;Rando OJ
The small RNA payload of mammalian sperm undergoes dramatic remodeling during development, as several waves of microRNAs and tRNA fragments are shipped to sperm during post-testicular maturation in the epididymis. Here, we take advantage of this developmental process to probe the function of the sperm RNA payload in preimplantation development. We generated zygotes via intracytoplasmic sperm injection (ICSI) using sperm obtained from the proximal (caput) vs. distal (cauda) epididymis, then characterized development of resulting embryos. Embryos generated using caput sperm significantly overexpress multiple regulatory factors throughout preimplantation development, and subsequently implant inefficiently and fail soon after implantation. Remarkably, microinjection of purified cauda-specific small RNAs into caput-derived embryos not only completely rescued preimplantation molecular defects, but also suppressed the postimplantation embryonic lethality phenotype. These findings reveal an essential role for small RNA remodeling during post-testicular maturation of mammalian sperm, and identify a specific preimplantation gene expression program responsive to sperm-delivered microRNAs. Conine et al. take advantage of RNA repertoire changes across sperm maturation to examine sperm RNA payload function in embryonic development. Embryos generated using proximal epididymis-derived sperm exhibit altered gene regulation and fail to implant. Microinjection of microRNAs normally gained in the distal epididymis is sufficient to rescue these defects.
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DOI：
10.1523/jneurosci.0914-13.2013
发表时间：
2013-05-22
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Rodgers AB;Morgan CP;Bronson SL;Revello S;Bale TL
通讯作者：
Bale TL
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
64.5
作者：
Rando OJ
通讯作者：
Rando OJ
影响因子：
25
作者：
Gapp, Katharina;Jawaid, Ali;Sarkies, Peter;Bohacek, Johannes;Pelczar, Pawel;Prados, Julien;Farinelli, Laurent;Miska, Eric;Mansuy, Isabelle M.
通讯作者：
Mansuy, Isabelle M.
影响因子：
2.8
作者：
Said, S;Han, MS;Niwa, K
通讯作者：
Niwa, K