Identification of Eya3 and TAC1 as long-day signals in the sheep pituitary.
Identification of Eya3 and TAC1 as long-day signals in the sheep pituitary.
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DOI：
10.1016/j.cub.2010.02.066
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发表时间：
2010-05-11
期刊：
影响因子：
--
通讯作者：
Loudon AS
中科院分区：
文献类型：
--
作者：
Dupré SM;Miedzinska K;Duval CV;Yu L;Goodman RL;Lincoln GA;Davis JR;McNeilly AS;Burt DD;Loudon AS
季节性繁殖的哺乳动物，如绵羊，利用由松果体激素褪黑激素的夜间分泌编码的光周期，作为驱动激素节律和使繁殖同步到一年中最佳时间的关键线索。褪黑激素直接作用于垂体的结节部（PT），调节促甲状腺激素（TSH）的表达，然后将信息传递回下丘脑以控制生殖回路。此外，第二个局部垂体内回路通过目前未表征的PT来源的称为tuberalin的低分子量肽控制季节性催乳素（PRL）释放。对鸟类的研究发现转录因子Eya 3是长光周期（LP）激活的第一个分子反应。使用阵列和原位杂交研究，我们显示Eya 3作为最强的LP激活基因在绵羊，揭示了一个共同的光周期的分子反应在鸟类和哺乳动物。我们还确定了TAC 1（编码速激肽物质P和神经激肽A; NKA）在绵羊PT内被LP强烈激活。我们表明，这些催乳素促分泌素作用于初级垂体细胞，因此是难以捉摸的PT表达的“tuberalin”季节性激素调节剂的候选人。
Seasonally breeding mammals such as sheep use photoperiod, encoded by the nocturnal secretion of the pineal hormone melatonin, as a critical cue to drive hormone rhythms and synchronise reproduction to the most optimal time of year. Melatonin acts directly on the pars tuberalis (PT) of the pituitary, regulating expression of thyrotropin (TSH) which then relays messages back to the hypothalamus to control reproductive circuits. In addition, a second local intra-pituitary circuit controls seasonal prolactin (PRL) release via a currently uncharacterised low molecular weight peptide(s) termed tuberalin(s) of PT origin. Studies in birds identified the transcription factor Eya3 as the first molecular response activated by long photoperiods (LP). Using arrays and in situ hybridization studies, we show Eya3 as the strongest LP activated gene in sheep, revealing a common photoperiodic molecular response in birds and mammals. We also identified TAC1 (encoding the tachykinins Substance P and Neurokinin A; NKA) to be strongly activated by LP within the sheep PT. We show that these PRL secretagogues act on primary pituitary cells, and are thus candidates for the elusive PT-expressed “tuberalin” seasonal hormone regulator.
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影响因子：
9.2
作者：
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通讯作者：
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影响因子：
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