The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.

The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.
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DOI:
10.1387/ijdb.130199bl
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发表时间:
2013
期刊:
The International journal of developmental biology
影响因子:
--
通讯作者:
--
中科院分区:
其他
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根癌农杆菌介导的植物遗传转化是植物生物学基础研究和应用研究的重要手段。为了成功感染,最终将其转移的DNA(T-DNA)整合到宿主基因组中,农杆菌依赖于与宿主植物因子的多重相互作用。广泛的研究已经揭示了在感染过程的所有主要步骤中的许多这样的相互作用:细菌毒力基因的激活,细胞-细胞接触和大分子从农杆菌转移到宿主细胞质,T-DNA和相关蛋白(T-复合物)的细胞内转运到宿主细胞核,T-复合物的分解,T-DNA整合和转移基因的表达。在所有这些过程中,农杆菌已经进化到控制甚至利用宿主植物防御反应的几条途径。这些农杆菌-宿主相互作用的研究大大提高了我们对许多基本细胞生物学过程的理解,并允许改进农杆菌作为生物技术基因转移工具的使用。
The genetic transformation of plants mediated by Agrobacterium tumefaciens represents an essential tool for both fundamental and applied research in plant biology. For a successful infection, culminating in the integration of its transferred DNA (T-DNA) into the host genome, Agrobacterium relies on multiple interactions with host-plant factors. Extensive studies have unraveled many of such interactions at all major steps of the infection process: activation of the bacterial virulence genes, cell-cell contact and macromolecular translocation from Agrobacterium to host cell cytoplasm, intracellular transit of T-DNA and associated proteins (T-complex) to the host cell nucleus, disassembly of the T-complex, T-DNA integration, and expression of the transferred genes. During all these processes, Agrobacterium has evolved to control and even utilize several pathways of host-plant defense response. Studies of these Agrobacterium-host interactions substantially enhance our understanding of many fundamental cellular biological processes and allow improvements in the use of Agrobacterium as a gene transfer tool for biotechnology.
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