Comparative Study of Tissue Affinity, Chemical Characteristics of Cultured and Natural Coral as a Bioabsorbable Scaffold
Comparative Study of Tissue Affinity, Chemical Characteristics of Cultured and Natural Coral as a Bioabsorbable Scaffold
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培养珊瑚和天然珊瑚作为生物可吸收支架的组织亲和力、化学特性的比较研究
DOI：
10.11223/jarde.14.164
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Tamura Isao
中科院分区：
文献类型：
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作者：
Matsuda Yoshifumi;Nishikawa Tetsunari;Okamura Tomoharu;Tominaga Kazuya;Wato Masahiro;Tabata Hajime;Umeda Makoto;Okusa Nobutaka;I. Koichi;Tanaka Akio;Tamura Isao
珊瑚是一种多孔材料，具有粗糙的表面和物理强度，可以有效地用作骨骼生长的支架。为了比较人工养殖珊瑚和天然珊瑚的生物相容性，我们在体外检测了它们的化学特性和组织亲和性。采用培养珊瑚和天然珊瑚（指状珊瑚），用氢氧化钠脱蛋白。将培养的和天然的珊瑚颗粒浸泡在含钙缓冲液（pH 6.0、pH 7.0、pH 8.0）中10天，然后测量缓冲液中的钙密度。测量不含这些颗粒的溶液中的钙密度作为对照组。人牙周膜成纤维细胞（HPLF）增殖。然后将培养的和天然的珊瑚颗粒接种在具有HPLF的威尔斯孔中。培养7 d后，用DAPI和5-乙炔基-2 '-脱氧尿苷（5-Ethynyl-2'-deoxyuridine，EdU）对HPLF进行染色，观察到细胞数量增加，细胞周期S期延长。培养没有这些颗粒的细胞作为对照。pH 6.0的缓冲液浸泡人工养殖珊瑚后，其钙离子浓度比浸泡天然珊瑚后增加得多。另一方面，与浸泡天然珊瑚相比，pH 8.0的接种培养珊瑚中的钙浓度降低。培养7天后，与天然珊瑚相比，由HPLF组成的细胞在培养的珊瑚颗粒周围密集地生长。因此，培养的珊瑚将提供生物可吸收和生物相容性支架。
Coral is a porous material with rough surface, and physical strength that could be effectively used as scaffolding for bone growth. To compare the bioabsorbility and biocompatibility between cultured and natural coral, we examined the chemical characteristics and tissue affinity in vitro. The cultured and natural coral (montipora digitata) deproteinized with NaOH was used. The cultured and natural coral particles were soaked in calcium containing buffers (pH 6.0, pH 7.0, pH 8.0) for 10 days, and the calcium density in the buffer was then measured. The calcium density in a solution without these particles was measured as the control group. Human periodontal ligament fibroblast (HPLF) was multiplied. The cultured and natural coral particles were then seeded in the wells with HPLF. After 7 days of culture, the HPLF were stained with DAPI and 5-Ethynyl-2'-deoxyuridine (EdU), and the increase in cell number and S phase of the cell cycle were observed. Cells without these particles were cultured as a control. The density of calcium in the pH 6.0 buffer which soaked the cultured coral increased more than that which soaked the natural coral. On the other hand, the calcium density in the pH 8.0 seeded cultured coral was decreased compared with that which soaked the natural coral. After 7 days of culture, cells consisting of HPLF grew densely around the cultured coral particles compared with the natural coral. Thus, the cultured coral would provide a bioabsorbable and biocompatible scaffold.
养殖珊瑚和天然珊瑚作为骨增量支架的物理和形态特征的比较研究。
DOI：
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发表时间：
2016
期刊：
J Oral Tissue Engin.
影响因子：
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作者：
Nishikawa T;Okamura T;Masuno K;Matsumoto H;Hirose M;Uemura N;Yasuda N;Hidaka M;Baba S;Imai K;Tanaka A.
通讯作者：
Tanaka A.
影响因子：
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作者：
Nishikawa, Tetsunari;Masuno, Kazuya;Tanaka, Akio
通讯作者：
Tanaka, Akio