Bioactive in situ crosslinkable polymer-peptide hydrogel for cell delivery to the intervertebral disc in a rat model.
Bioactive in situ crosslinkable polymer-peptide hydrogel for cell delivery to the intervertebral disc in a rat model.
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生物活性原位交联聚合物-肽水凝胶用于大鼠模型椎间盘细胞递送。
DOI：
10.1016/j.actbio.2021.06.045
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发表时间：
2021-09-01
影响因子：
9.7
通讯作者：
Setton, Lori A.
中科院分区：
文献类型：
--
作者：
Barcellona, Marcos N.;Speer, Julie E.;Jing, Liufang;Patil, Deepanjali S.;Gupta, Munish C.;Buchowski, Jacob M.;Setton, Lori A.
Degeneration of the intervertebral disc (IVD) is associated with significant biochemical and morphological changes that include a loss of disc height, decreased water content and decreased cellularity. Cell delivery has been widely explored as a strategy to supplement the nucleus pulposus (NP) region of the degenerated IVD in both pre-clinical and clinical trials, using progenitor or primary cell sources. We previously demonstrated an ability for a polymer-peptide hydrogel, serving as a culture substrate, to promote adult NP cells to undergo a shift from a degenerative fibroblast-like state to a juvenile-like NP phenotype. In the current study, we evaluate the ability for this peptide-functionalized hydrogel to serve as a bioactive system for cell delivery, retention and preservation of a biosynthetic phenotype for primary IVD cells delivered to the rat caudal disc in an anular puncture degeneration model. Our data suggest that encapsulation of adult degenerative human NP cells in a stiff formulation of the hydrogel functionalized with laminin-mimetic peptides IKVAV and AG73 can promote cell viability and increased biosynthetic activity for this population in 3D culture in vitro. Delivery of the peptide-functionalized biomaterial with primary rat cells to the degenerated IVD supported NP cell retention and NP-specific protein expression in vivo, and promoted improved disc height index (DHI) values and endplate organization compared to untreated degenerated controls. The results of this study suggest the physical cues of this peptide-functionalized hydrogel can serve as a supportive carrier for cell delivery to the IVD.
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影响因子：
14
作者：
Barcellona MN;Speer JE;Fearing BV;Jing L;Pathak A;Gupta MC;Buchowski JM;Kelly M;Setton LA
通讯作者：
Setton LA
影响因子：
168.9
作者：
Freemont, AJ;Peacock, TE;Jayson, MIV
通讯作者：
Jayson, MIV
影响因子：
3.7
作者：
Gilchrist CL;Darling EM;Chen J;Setton LA
通讯作者：
Setton LA
影响因子：
4.1
作者：
Bowles, Robby D.;Williams, Rebecca M.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
2.8
作者：
Cloyd, Jordan M.;Malhotra, Neil R.;Elliott, Dawn M.
通讯作者：
Elliott, Dawn M.