Control of adhesive ligand density for modulation of nucleus pulposus cell phenotype.
Control of adhesive ligand density for modulation of nucleus pulposus cell phenotype.
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DOI：
10.1016/j.biomaterials.2020.120057
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发表时间：
2020-08
期刊：
影响因子：
14
通讯作者：
Setton LA
中科院分区：
文献类型：
--
作者：
Barcellona MN;Speer JE;Fearing BV;Jing L;Pathak A;Gupta MC;Buchowski JM;Kelly M;Setton LA
已经观察到髓核细胞随着年龄和成熟从其脊索样幼年表型转变为更像成纤维细胞的状态。已经证明，在软的（< 1 kPa）全长含层粘连蛋白的水凝胶基质上培养退行性成人髓核细胞促进了与幼年髓核细胞表型相关的一组标志物的水平增加。在当前的工作中，我们观察到使用用短的层粘连蛋白模拟肽序列官能化的软聚合物基底来概括由软的、全长的含层粘连蛋白的材料引起的行为的能力。此外，我们的工作表明，通过控制肽密度较硬的基板上模仿软系统的功能的能力。具体地，结果表明，更硬的聚合物-肽水凝胶基质可用于通过减少粘附配体呈递来促进髓核细胞的更幼稚样表型的表达。在这里，我们展示了如何结合粘合剂配体介绍聚合物刚度可以控制，以支持髓核细胞的表型和生物合成。
Cells of the nucleus pulposus have been observed to undergo a shift from their notochordal-like juvenile phenotype to a more fibroblast-like state with age and maturation. It has been demonstrated that culture of degenerative adult human nucleus pulposus cells upon soft (< 1 kPa) full length laminin-containing hydrogel substrates promotes increased levels of a panel of markers associated with the juvenile nucleus pulposus cell phenotype. In the current work, we observed an ability to use soft polymeric substrates functionalized with short laminin-mimetic peptide sequences to recapitulate the behaviors elicited by soft, full-length laminin containing materials. Furthermore, our work suggests an ability to mimic features of soft systems through control of peptide density upon stiffer substrates. Specifically, results suggest that stiffer polymer-peptide hydrogel substrates can be used to promote the expression of a more juvenile-like phenotype for cells of the nucleus pulposus by reducing adhesive ligand presentation. Here we show how polymer stiffness combined with adhesive ligand presentation can be controlled to be supportive of nucleus pulposus cell phenotype and biosynthesis.
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影响因子：
2.7
作者：
Choi H;Johnson ZI;Risbud MV
通讯作者：
Risbud MV
DOI：
10.1073/pnas.1214100110
发表时间：
2013-06-18
影响因子：
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影响因子：
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作者：
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通讯作者：
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