Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN.
Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN.
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DOI：
10.1016/j.virol.2013.07.019
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发表时间：
2013-11
期刊：
影响因子：
3.7
通讯作者：
Morris, Lynn
中科院分区：
文献类型：
--
作者：
Alexandre, Kabamba B.;Moore, Penny L.;Nonyane, Molati;Gray, Elin S.;Ranchobe, Nthabeleng;Chakauya, Ereck;McMahon, James B.;O'Keefe, Barry R.;Chikwamba, Rachel;Morris, Lynn
关键词：
我们检测了HIV-1亚型C对抑制凝集素griffithsin （GRFT）、cyanovirin-N （CV-N）和scytovirin （SVN）产生耐药性的能力，这些凝集素在gp120上结合多种富含甘露糖的聚糖。在这些凝集素浓度不断升高的条件下培养的四株原代HIV-1菌株对其50%抑制浓度的耐受性增加了2至12倍。gp120的序列分析显示，大多数基因缺失1 ~ 5个富含甘露糖的聚糖。C2区230、234、241、289位点和C3-C4区339、392、448位点的糖基化位点受到影响。此外，4株分离株中有3株在V4区发现了多达5个氨基酸的缺失和插入。这些数据表明gp120上糖基化位点的缺失以及V4中聚糖的重排是HIV-1亚型C从GRFT、CV-N和SVN中逃逸的机制。
We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN.
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DOI：
10.1038/nri2569
发表时间：
2009-07
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Geijtenbeek TB;Gringhuis SI
通讯作者：
Gringhuis SI
影响因子：
5.4
作者：
Balzarini, J;Van Laethem, K;Schols, D
通讯作者：
Schols, D
影响因子：
3.6
作者：
Balzarini, J;Van Laethem, K;Schols, D
通讯作者：
Schols, D
影响因子：
1.5
作者：
Choge, Isaac;Cilliers, Tonie;Morris, Lynn
通讯作者：
Morris, Lynn
国际艾滋病毒中和试验比较网络：NeutNet 报告。
影响因子：
3.7
作者：
Fenyo, Eva Maria;Heath, Alan;Dispinseri, Stefania;Holmes, Harvey;Lusso, Paolo;Zolla-Pazner, Susan;Donners, Helen;Heyndrickx, Leo;Alcami, Jose;Bongertz, Vera;Jassoy, Christian;Malnati, Mauro;Montefiori, David;Moog, Christiane;Morris, Lynn;Osmanov, Saladin;Polonis, Victoria;Sattentau, Quentin;Schuitemaker, Hanneke;Sutthent, Ruengpung;Wrin, Terri;Scarlatti, Gabriella
通讯作者：
Scarlatti, Gabriella