International network for comparison of HIV neutralization assays: the NeutNet report.
International network for comparison of HIV neutralization assays: the NeutNet report.
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国际艾滋病毒中和试验比较网络：NeutNet 报告。
DOI：
10.1371/journal.pone.0004505
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Scarlatti, Gabriella
中科院分区：
文献类型：
--
作者：
Fenyo, Eva Maria;Heath, Alan;Dispinseri, Stefania;Holmes, Harvey;Lusso, Paolo;Zolla-Pazner, Susan;Donners, Helen;Heyndrickx, Leo;Alcami, Jose;Bongertz, Vera;Jassoy, Christian;Malnati, Mauro;Montefiori, David;Moog, Christiane;Morris, Lynn;Osmanov, Saladin;Polonis, Victoria;Sattentau, Quentin;Schuitemaker, Hanneke;Sutthent, Ruengpung;Wrin, Terri;Scarlatti, Gabriella
中和抗体评估在人类免疫缺陷病毒1型（HIV-1）疫苗开发中发挥着核心作用，但目前尚不清楚哪种检测试剂盒或检测试剂盒组合将提供可靠的保护相关措施。为了解决这个问题，组织了一个涉及18名独立参与者的国际合作（NeutNet），以比较不同的检测方法。每个实验室评价了给定浓度范围内的4种中和试剂（TriMab、447 - 52D、4E10、sCD4），以对抗代表广泛遗传亚型和表型的11种病毒。总共比较了16种不同的测定。这些测定利用外周血单核细胞（PBMCs）中产生的未克隆病毒（病毒感染性测定，VI测定），或在293 T细胞中产生的来自分子克隆或未克隆病毒的Env假型（gp160）衍生物（PSV测定）。靶细胞包括PBMC和单循环或多循环感染形式的基因工程细胞系。通过使用一系列测定读数来定量感染，所述测定读数包括细胞外或细胞内p24抗原检测、RNA定量以及荧光素酶和β-半乳糖苷酶报告基因表达。PSV测定法通常比VI测定法更灵敏，但根据所用病毒和抑制剂存在重要差异。例如，对于TriMab，PSV中的平均IC50始终低于VI试验。然而，使用4E10或sCD 4，在VI试验中中和某些病毒的IC50低于PSV试验。对于某些病毒，PSV的实验室间一致性略好于VI检测，但对于其他病毒，实验室间一致性有限，并取决于病毒和中和试剂。NeutNet项目证明了检测灵敏度的明显差异，这取决于中和试剂和病毒。没有单一试验能够检测整个中和活性谱。由于尚不清楚哪种体外试验与体内保护相关，因此建议采用一系列中和试验进行疫苗评价。
Neutralizing antibody assessments play a central role in human immunodeficiency virus type-1 (HIV-1) vaccine development but it is unclear which assay, or combination of assays, will provide reliable measures of correlates of protection. To address this, an international collaboration (NeutNet) involving 18 independent participants was organized to compare different assays. Each laboratory evaluated four neutralizing reagents (TriMab, 447-52D, 4E10, sCD4) at a given range of concentrations against a panel of 11 viruses representing a wide range of genetic subtypes and phenotypes. A total of 16 different assays were compared. The assays utilized either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (virus infectivity assays, VI assays), or their Env-pseudotyped (gp160) derivatives produced in 293T cells (PSV assays) from molecular clones or uncloned virus. Target cells included PBMC and genetically-engineered cell lines in either a single- or multiple-cycle infection format. Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression. PSV assays were generally more sensitive than VI assays, but there were important differences according to the virus and inhibitor used. For example, for TriMab, the mean IC50 was always lower in PSV than in VI assays. However, with 4E10 or sCD4 some viruses were neutralized with a lower IC50 in VI assays than in the PSV assays. Inter-laboratory concordance was slightly better for PSV than for VI assays with some viruses, but for other viruses agreement between laboratories was limited and depended on both the virus and the neutralizing reagent. The NeutNet project demonstrated clear differences in assay sensitivity that were dependent on both the neutralizing reagent and the virus. No single assay was capable of detecting the entire spectrum of neutralizing activities. Since it is not known which in vitro assay correlates with in vivo protection, a range of neutralization assays is recommended for vaccine evaluation.
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DOI：
10.1016/j.bbrc.2006.07.163
发表时间：
2006-09-29
影响因子：
3.1
作者：
Choudhry, Vidita;Zhang, Mei-Yun;Dimitrov, Dimiter S.
通讯作者：
Dimitrov, Dimiter S.
影响因子：
5.4
作者：
Gorny, MK;Revesz, K;Zolla-Pazner, S
通讯作者：
Zolla-Pazner, S
影响因子：
3.1
作者：
Heyndrickx, Leo;Vermoesen, Tine;Vanham, Guido
通讯作者：
Vanham, Guido
影响因子：
120.7
作者：
BELSHE, RB;GRAHAM, BS;FAST, P
通讯作者：
FAST, P
影响因子：
3.8
作者：
Carotenuto, P;Looij, D;Goudsmit, J
通讯作者：
Goudsmit, J