Soft fibrin gels promote selection and growth of tumorigenic cells.
Soft fibrin gels promote selection and growth of tumorigenic cells.
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DOI：
10.1038/nmat3361
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发表时间：
2012-07-01
期刊：
影响因子：
41.2
通讯作者：
中科院分区：
文献类型：
--
作者：
通过依赖干细胞标记物的传统方法来鉴定干细胞样癌细胞通常是不可靠的。我们开发了一种机械方法，通过在硬度为~ 100pa的纤维蛋白基质中培养单个癌细胞来选择致瘤细胞。当在这些凝胶中培养时，原代人类癌细胞或来自小鼠或人类癌细胞系的单个癌细胞在几天内生长成类似胚胎干细胞菌落的单个圆形菌落。将10或100个纤维蛋白培养的细胞皮下或静脉注射到同质或严重联合免疫缺陷小鼠中，可导致在注射部位或远端肺器官形成实体肿瘤，其效率远高于使用常规表面标记方法选择的对照癌细胞，或在常规刚性培养皿或软凝胶上培养的对照癌细胞。值得注意的是，只有10个这样的细胞能够在野生型非同基因小鼠的肺部存活并形成肿瘤。
The identification of stem-cell-like cancer cells through conventional methods that depend on stem-cell markers is often unreliable. We developed a mechanical method of selecting tumourigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem-cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe-combined-immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as 10 such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice.
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影响因子：
3.4
作者：
Poh, Yeh-Chuin;Chowdhury, Farhan;Wang, Ning
通讯作者：
Wang, Ning
影响因子：
3.7
作者：
Chowdhury F;Li Y;Poh YC;Yokohama-Tamaki T;Wang N;Tanaka TS
通讯作者：
Tanaka TS
影响因子：
64.8
作者：
Quintana, Elsa;Shackleton, Mark;Sabel, Michael S.;Fullen, Douglas R.;Johnson, Timothy M.;Morrison, Sean J.
通讯作者：
Morrison, Sean J.
DOI：
10.1163/156856208786052380
发表时间：
2008-01-01
影响因子：
3.6
作者：
Hong, Helen;Stegemann, Jan P.
通讯作者：
Stegemann, Jan P.
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM