Evidence that reduced nitric oxide signal contributes to cutaneous microvascular dysfunction in peripheral arterial disease.
Evidence that reduced nitric oxide signal contributes to cutaneous microvascular dysfunction in peripheral arterial disease.
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有证据表明，一氧化氮信号减少会导致外周动脉疾病中的皮肤微血管功能障碍。
DOI：
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发表时间：
2015
影响因子：
2.1
通讯作者：
G. Tew
中科院分区：
文献类型：
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作者：
G. Hodges;S. Nawaz;G. Tew
外周动脉疾病（PAD）与皮肤微血管功能障碍和受累下肢动脉溃疡风险增加相关。本研究旨在探讨一氧化氮（NO）在PAD患者皮肤微血管功能障碍中的作用。使用激光多普勒血流仪，我们测量了5名单侧症状性PAD患者和10名年龄匹配的健康对照者的皮肤血流量（SkBF），在基线和局部皮肤加热至42°C 40分钟期间，1）未治疗的小腿部位，2）用20 mM N（G）-硝基-L-精氨酸甲酯（L-NAME）治疗的小腿部位抑制NO合酶活性。SkBF表示为激光多普勒通量（LDF），并标准化为通过局部加热至44 ℃并同时输注56 mM硝普钠实现的最大LDF（%LDF（max））。使用皮内微透析给予药物和对照治疗（乳酸林格氏液）。PAD患者患病肢体（最大70.3± 13.6%）对未治疗皮肤部位局部皮肤加温的平台LDF反应显著（P<0.05）低于未患病对侧肢体（最大85.0± 10.2%）和对照参与者观察到的反应（最大89.0± 5.2%）。PAD患者患肢中NO对平台SkBF反应的贡献倾向于较低（分别为45.1±16.4% vs 56.1±10.7% [P=0.12]和55.4±11.5% [P=0.13]）。结果表明，PAD损害下游皮肤微血管舒张功能，微血管功能障碍可能是解释，至少部分，通过减少NO信号。
Peripheral arterial disease (PAD) is associated with cutaneous microvascular dysfunction and an increased risk of arterial ulceration in the affected lower-limb(s). The purpose of this study was to investigate the role of nitric oxide (NO) in cutaneous microvascular dysfunction in patients with PAD. Using laser-Doppler flowmetry, we measured skin blood flow (SkBF) in 5 patients with unilateral symptomatic PAD and 10 age-matched healthy controls at baseline and during 40 min of local skin heating to 42°C at 1) untreated lower-leg sites, and 2) lower-leg sites treated with 20 mM N(G)-nitro-L-arginine methyl ester (L-NAME) to inhibit NO synthase activity. SkBF was expressed as laser-Doppler flux (LDF) and normalized to maximal LDF (%LDF(max)) achieved through localized heating to 44°C and concomitant infusion of 56 mM sodium nitroprusside. Pharmacological agents and control treatments (lactated Ringer's) were administered using intradermal microdialysis. The plateau LDF response to local skin warming at the untreated skin sites was significantly (P<0.05) lower in the diseased limb of the PAD patients (70.3±13.6 %max) compared to the non-diseased contralateral limb (85.0±10.2 %max) and the response observed for the control participants (89.0±5.2 %max). The NO contribution to the plateau SkBF response tended to be lower in the diseased limb of the PAD patients (45.1±16.4% versus 56.1±10.7% [P=0.12] and 55.4±11.5% [P=0.13], respectively). The results suggest that PAD impairs downstream cutaneous microvascular vasodilatory function and that the microvascular dysfunction is probably explained, at least in part, by a reduced NO signal.
2 型糖尿病身体发热期间的皮肤血流量和一氧化氮。
DOI：
10.1152/japplphysiol.91289.2008
发表时间：
2009
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
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作者：
Sokolnicki,LynnA;Strom,NicholasA;Roberts,ShellyK;Kingsley-Berg,ShirleyA;Basu,Ananda;Charkoudian,Nisha
通讯作者：
Charkoudian,Nisha
DOI：
10.2741/s105
发表时间：
2010-06
期刊：
Frontiers in bioscience
影响因子：
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作者：
John M. Johnson;D. Kellogg
通讯作者：
John M. Johnson;D. Kellogg