Characterization of carbonate fraction of the Atlantic bluefin tuna fin spine bone matrix for stable isotope analysis
Characterization of carbonate fraction of the Atlantic bluefin tuna fin spine bone matrix for stable isotope analysis
复制标题
大西洋蓝鳍金枪鱼鳍脊柱骨基质碳酸盐部分的表征，用于稳定同位素分析
DOI：
10.7717/peerj.7176
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发表时间：
2019
期刊：
影响因子：
2.7
通讯作者：
H. Arrizabalaga
中科院分区：
文献类型：
--
作者：
P. Luque;M. B. Sánchez;Alfredo Sarmiento;H. Murua;H. Arrizabalaga
鱼耳石（耳骨）的矿物成分是文石碳酸钙（CaCO3），使这种结构成为测量生物碳和氧稳定同位素的首选样品，以解决鱼类生态学和渔业科学中的基本问题。其主要缺点是去除耳石需要牺牲标本，这对于濒危和具有商业价值的物种，如大西洋蓝鳍金枪鱼（Thunnus thynnus）（ABFT）来说尤其不切实际。本研究探讨是否适合使用第一背鳍脊骨的ABFT作为一个非致命的替代耳石分析或作为一个补充的硬结构。收集新鲜捕获的ABFT的鳍刺以鉴定矿物基质中的碳酸根离子（即，羟基磷灰石），并确定晶格内碳酸盐取代的性质，这对于碳酸盐的氧和碳稳定同位素的正确测量和生态解释至关重要。通过X射线光电子能谱（XPS）、拉曼光谱和傅里叶变换红外光谱（FTIR）分析鳍棘切片。XPS测量分析显示Ca、O和P（构成羟基磷灰石的三种组成元素）的信号。拉曼和FTIR技术显示了羟基磷灰石基质中碳酸根离子的证据，其中IR光谱对于识别B型碳酸根取代是最有效的，如在v2 CO32 −域中在10872 cm − 1处的碳酸根谱带所示。本研究的结果证实了碳酸根离子的存在下，在矿物基质的鳍棘骨的ABFT，显示了使用这种钙化结构的稳定同位素分析的可行性。总体而言，我们的研究结果将促进保护具有商业价值和濒危/受保护鱼类的新方法，并将开辟新的研究途径来改善渔业管理和物种保护策略。
The mineral component of fish otoliths (ear bones), which is aragonitic calcium carbonate (CaCO3), makes this structure the preferred sample choice for measuring biological carbon and oxygen-stable isotopes in order to address fundamental questions in fish ecology and fisheries science. The main drawback is that the removal of otoliths requires sacrificing the specimen, which is particularly impractical for endangered and commercially valuable species such as Atlantic bluefin tuna (Thunnus thynnus) (ABFT). This study explores the suitability of using the first dorsal fin spine bone of ABFT as a non-lethal alternative to otolith analysis or as a complementary hard structure. The fin spines of freshly caught ABFT were collected to identify carbonate ions within the mineral matrix (i.e., hydroxyapatite) and to determine the nature of the carbonate substitution within the crystal lattice, knowledge which is crucial for correct measurement and ecological interpretation of oxygen and carbon stable isotopes of carbonates. Fin spine sections were analyzed via X-ray Photoelectron Spectroscopy (XPS), Raman Spectroscopy, and Fourier Transform InfraRed (FTIR). The XPS survey analysis showed signals of Ca, O, and P (three compositional elements that comprise hydroxyapatite). The Raman and FTIR techniques showed evidence of carbonate ions within the hydroxyapatite matrix, with the IR spectra being the most powerful for identifying the type B carbonate substitution as shown by the carbonate band in the v2 CO32− domain at ∼872 cm−1. The results of this study confirmed the presence of carbonate ions within the mineral matrix of the fin spine bone of ABFT, showing the feasibility of using this calcified structure for analysis of stable isotopes. Overall, our findings will facilitate new approaches to safeguarding commercially valuable and endangered/protected fish species and will open new research avenues to improve fisheries management and species conservation strategies.
影响因子：
7.4
作者：
Lu, HB;Campbell, CT;Ratner, BD
通讯作者：
Ratner, BD