Chromatin and microtubule organization during premeiotic, meiotic and early postmeiotic stages of Drosophila melanogaster spermatogenesis.
Chromatin and microtubule organization during premeiotic, meiotic and early postmeiotic stages of Drosophila melanogaster spermatogenesis.
复制标题
果蝇精子发生的减数分裂前、减数分裂和减数分裂后早期的染色质和微管组织。
作者：
G. Cenci;S. Bonaccorsi;Claudio Pisano;F. Vernì;M. Gatti
采用甲醇/丙酮固定程序固定果蝇幼虫和蛹睾丸，细胞形态得到良好保存；通过相差光学观察的固定细胞表现出活体材料中可见的大部分结构细节。用抗微管蛋白抗体和 Hoechst 33258 处理固定的睾丸制剂，以选择性地对微管和 DNA 进行染色。细胞形态、染色质和微管组织的综合分析可以对生殖细胞增殖、精母细胞发育、减数分裂和精子细胞分化的早期阶段进行精细的细胞学解剖。我们特别强调精母细胞生长阶段和减数分裂，对这些过程的描述比以前报道的要详细得多。此外，通过溴脱氧尿苷掺入实验，我们能够证明减数分裂前 DNA 合成在精母细胞生长过程中发生得很早。
Larval and pupal testes of Drosophila melanogaster were fixed with a methanol/acetone fixation procedure that results in good preservation of cell morphology; fixed cells viewed by phase-contrast optics exhibit most of the structural details that can be seen in live material. Fixed testis preparations were treated with anti-tubulin antibodies and Hoechst 33258 to selectively stain microtubules and DNA. The combined analysis of cell morphology, chromatin and microtubule organization allowed a fine cytological dissection of gonial cell multiplication, spermatocyte development, meiosis and the early stages of spermatid differentiation. We placed special emphasis on the spermatocyte growth phase and the meiotic divisions, providing a description of these processes that is much more detailed than those previously reported. In addition, by means of bromo-deoxyuridine incorporation experiments, we were able to demonstrate that premeiotic DNA synthesis occurs very early during spermatocyte growth.
影响因子：
2.7
作者：
KIMBLE, M;INCARDONA, JP;RAFF, EC
通讯作者：
RAFF, EC