Internalization of C60 fullerenes into cancer cells with accumulation in the nucleus via the nuclear pore complex.
Internalization of C60 fullerenes into cancer cells with accumulation in the nucleus via the nuclear pore complex.
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DOI：
10.1016/j.biomaterials.2011.12.043
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发表时间：
2012-04
期刊：
影响因子：
14
通讯作者：
Curley, Steven A.
中科院分区：
文献类型：
--
作者：
Raoof, Mustafa;Mackeyev, Yuri;Cheney, Matthew A.;Wilson, Lon J.;Curley, Steven A.
A highly water-soluble, non-ionic, and non-cytotoxic fullerene malonodiserinolamide-derivatized fullerene C60 (C60-ser) is under investigation as a potential nanovector to deliver biologic and cancer drugs across biological barriers. Using laser-scanning confocal microscopy and flow cytometry, we find that PF-633 fluorophore conjugated C60-ser nanoparticles (C60-serPF) are internalized within living cancer cells in association with serum proteins through multiple energy-dependent pathways, and escape endocytotic vesicles to eventually localize and accumulate in the nucleus of the cells through the nuclear pore complex. Furthermore, in a mouse model of liver cancer, the C60-serPF conjugate is detected in most tissues, permeating through the altered vasculature of the tumor and the tightly-regulated blood brain barrier while evading the reticulo-endothelial system.
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