Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida.
Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida.
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黄单胞菌的致病性、表型和分子特征。
DOI：
10.1099/ijsem.0.002189
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发表时间：
2017
影响因子：
2.8
通讯作者：
D. Studholme
中科院分区：
文献类型：
--
作者：
J. Vicente;Steve D. Rothwell;E. Holub;D. Studholme
本文描述了从产于美国佛罗里达的豆瓣菜（Nasturtium officinale）病叶中分离的黄单胞菌属（Xanthomonas）两个新种。一种菌株对豆瓣菜具有致病性，但对包括一系列十字花科植物在内的其他物种不具有致病性;其他菌株对任何测试植物都不具有致病性。Biolog碳源利用测试数据和16 S和gyrB位点的核苷酸序列数据表明，致病性和非致病性菌株都与先前描述的黄单胞菌属物种有关，但又不同。多基因座序列分析和豆瓣菜两个菌株基因组平均核苷酸同一性（ANI）的全基因组比较显示，这些菌株是不同的，与所有其他已知物种的ANI低于95%;非致病性菌株WHRI 8848接近Xanthomonascassavae（ANI为93.72%），而致病性菌株WHRI 8853接近包括Xanthomonasvesicatoria（ANI ≤ 90.25%）在内的一个大型物种分支。 基于这些结果，我们提出这两个菌株代表新的黄单胞菌属物种，分别命名为黄单胞菌flororescensis sp. nov.（模式菌株WHRI 8848=ATCC TSD-60=LMG 29665=NCPPB 4601）和黄单胞菌nov.（模式菌株WHRI 8853=ATCC TSD-61=LMG 29666=NCPPB 4600）。西洋菜中是否存在非致病性黄单胞菌及其与致病性黄单胞菌的互作有待进一步研究。虽然新的致病物种的重要性尚待确定，但它引起的细菌性疾病对豆瓣菜生产构成威胁，应监测其分布情况。
We describe two new species of the genus Xanthomonas, represented by yellow mucoid bacterial strains isolated from diseased leaves of watercress (Nasturtium officinale) produced in Florida, USA. One strain was pathogenic on watercress, but not in other species including a range of brassicas; other strains were not pathogenic in any of the tested plants. Data from Biolog carbon source utilization tests and nucleotide sequence data from 16S and gyrB loci suggested that both pathogenic and non-pathogenic strains were related to, yet distinct from, previously described Xanthomonas species. Multilocus sequence analysis and whole genome-wide comparisons of the average nucleotide identity (ANI) of genomes of two strains from watercress showed that these are distinct and share less than 95 % ANI with all other known species; the non-pathogenic strain WHRI 8848 is close to Xanthomonascassavae (ANI of 93.72 %) whilst the pathogenic strain WHRI 8853 is close to a large clade of species that includes Xanthomonasvesicatoria (ANI ≤90.25 %). Based on these results, we propose that both strains represent new Xanthomonas species named Xanthomonas floridensis sp. nov. (type strain WHRI 8848=ATCC TSD-60=ICMP 21312=LMG 29665=NCPPB 4601) and Xanthomonas nasturtii sp. nov. (type strain WHRI 8853=ATCC TSD-61=ICMP 21313=LMG 29666=NCPPB 4600), respectively. The presence of non-pathogenic Xanthomonas strains in watercress and their interaction with pathogenic strains needs to be further investigated. Although the importance of the new pathogenic species is yet to be determined, the bacterial disease that it causes constitutes a threat to watercress production and its distribution should be monitored.
影响因子：
64.8
作者：
Bai, Yang;Mueller, Daniel B.;Schulze-Lefert, Paul
通讯作者：
Schulze-Lefert, Paul
影响因子：
14.9
作者：
Delcher, AL;Phillippy, A;Salzberg, SL
通讯作者：
Salzberg, SL