Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis.
Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis.
复制标题
铁氧还蛋白-NADP( )氧化还原酶对高盐胁迫条斑紫菜循环电子传递的调节
DOI：
10.3389/fpls.2018.01092
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Yu B;Niu J;Feng J;Xu M;Xie X;Gu W;Gao S;Wang G
条斑甲藻能够在低潮时的严重失水条件下存活，是研究潮间带海藻对特殊极端环境适应机制的理想物种。在这项研究中，我们确定了高盐度对光合作用的影响，利用周围的藻类组织增加盐度。电子传递速率ETR（I）和ETR（II）均随盐度的增加而持续下降。然而，这些因素之间的差异保持相对稳定，与对照组相似。抑制剂实验表明，至少有三种不同的循环电子传递途径。在高盐条件下，NAD（P）H可作为内源电子供体还原Py的质体醌库。yezoensis。基于这些发现，我们接下来通过克隆铁氧还蛋白-NADP+氧化还原酶（FNR）的基因（HM 370553）来研究这些不同的循环电子传递（CET）途径是如何协调的。构建了系统发育树，并对不同FNR之间的进化关系进行了评价。结果表明，Py.条斑藻FNR与蓝藻FNR的亲缘关系较近。实时聚合酶链反应和蛋白质印迹结果均表明，在90-120‰盐度下，该酶表达上调。由于生物体内结构与功能的相关性，Py.条斑紫菜FNR参与了盐胁迫下NAD（P）H依赖的Fd+还原反应。因此，通过FNR桥接不同供体之间的连接，根据不同的代谢需求，电子被引导到不同的路线。这将使叶绿体中的电子传递变得更加灵活，并有助于Py的驯化。yezoensis的极端多变的环境中的潮间带。
Pyropia yezoensis can survive the severe water loss that occurs during low tide, making it an ideal species to investigate the acclimation mechanism of intertidal seaweed to special extreme environments. In this study, we determined the effects of high salinity on photosynthesis using increasing salinity around algal tissues. Both electron transport rates, ETR (I) and ETR (II), showed continuous decreases as the salinity increased. However, the difference between these factors remained relatively stable, similar to the control. Inhibitor experiments illustrated that there were at least three different cyclic electron transport pathways. Under conditions of severe salinity, NAD(P)H could be exploited as an endogenous electron donor to reduce the plastoquinone pool in Py. yezoensis. Based on these findings, we next examined how these different cyclic electron transport (CETs) pathways were coordinated by cloning the gene (HM370553) for ferredoxin-NADP+ oxidoreductase (FNR). A phylogenetic tree was constructed, and the evolutionary relationships among different FNRs were evaluated. The results indicated that the Py. yezoensis FNR showed a closer relationship with cyanobacterial FNR. The results of both real-time polymerase chain reaction and western blotting showed that the enzyme was upregulated under 90–120‰ salinity. Due to the structure-function correlations in organism, Py. yezoensis FNR was proposed to be involved in NAD(P)H-dependent Fd+ reduction under severe salinity conditions. Thus, through the connection between different donors bridged by FNR, electrons were channeled toward distinct routes according to the different metabolic demands. This was expected to make the electron transfer in the chloroplasts become more flexible and to contribute greatly to acclimation of Py. yezoensis to the extreme variable environments in the intertidal zone.
登录
查看更多内容
影响因子：
3.7
作者：
Mosebach L;Heilmann C;Mutoh R;Gäbelein P;Steinbeck J;Happe T;Ikegami T;Hanke G;Kurisu G;Hippler M
通讯作者：
Hippler M
影响因子：
3.1
作者：
EILERS, PHC;PEETERS, JCH
通讯作者：
PEETERS, JCH
DOI：
10.1016/s0044-328x(81)80100-6
发表时间：
1981-01-01
期刊：
ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE
影响因子：
--
作者：
FORK, DC;OQUIST, G
通讯作者：
OQUIST, G
影响因子：
4.8
作者：
Hubbard, PA;Shen, AL;Kim, JJP
通讯作者：
Kim, JJP
影响因子：
4.9
作者：
Endo, T;Asada, K
通讯作者：
Asada, K