Sperm cryopreservation during the SARS-CoV-2 pandemic.
Sperm cryopreservation during the SARS-CoV-2 pandemic.
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DOI：
10.1007/s40618-020-01438-8
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发表时间：
2021-05
影响因子：
5.4
通讯作者：
Lombardo F
中科院分区：
文献类型：
--
作者：
Paoli D;Pallotti F;Nigro G;Aureli A;Perlorca A;Mazzuti L;Di Carlo D;Turriziani O;Lenzi A;Lombardo F
精子冷冻保存是接受性腺毒性治疗的患者的基本管理。由于SARS-CoV-2阳性冷冻保存的样本可能对受精和胚胎安全性产生未知影响，因此人们对SARS-CoV-2及其对睾丸的潜在影响越来越关注。因此，本研究旨在通过评估SARS-CoV-2暴露风险和精液样本的病毒RNA检测，分析意大利大流行爆发后癌症患者精子冷冻保存的安全性。我们招募了10名在意大利封锁期间（2020年3月11日至5月4日）转介到我们精子库的癌症患者（平均年龄30.5 ± 9.6岁），他们没有接受过SARS-CoV-2检测的鼻咽拭子。对患者进行了关于其接触COVID-19的问卷调查，并采集了精液样本。冷冻保存前，使用QIAamp病毒RNA试剂盒（Qiagen）从150 μl等份精液中提取SARS-CoV-2 RNA，并通过靶向E和S基因的真实的时间RT PCR系统（RealStar SARS-CoV 2 RT PCR，Altona Diagnostics）扩增。问卷调查和医学访谈显示，所有患者均无症状，且既往未与COVID-19感染者接触。所有精液样本均为SARS-CoV-2 RNA阴性。这一初步评估表明，对无症状癌症患者精液中不存在SARS-CoV-2的彻底评估（特别是在多学科团队的背景下）和分子确认可能有助于确保精子冷冻保存的安全性。
Sperm cryopreservation is fundamental in the management of patients undergoing gonadotoxic treatments. Concerns have risen in relation to SARS-CoV-2 and its potential for testicular involvement, since SARS-CoV-2-positive cryopreserved samples may have unknown effects on fertilization and embryo safety. This study therefore aimed to analyze the safety of sperm cryopreservation for cancer patients after the onset of the pandemic in Italy, through assessment of the risk of SARS-CoV-2 exposure and viral RNA testing of semen samples. We recruited 10 cancer patients (mean age 30.5 ± 9.6 years) referred to our Sperm Bank during the Italian lockdown (from March 11th to May 4th 2020) who had not undergone a nasopharyngeal swab for SARS-CoV-2 testing. Patients were administered a questionnaire on their exposure to COVID-19, and semen samples were taken. Before cryopreservation, SARS-CoV-2 RNA was extracted from a 150 µl aliquot of seminal fluid in toto using QIAamp viral RNA kit (Qiagen) and amplified by a real time RT PCR system (RealStar SARS-CoV2 RT PCR, Altona Diagnostics) targeting the E and S genes. The questionnaire and medical interview revealed that all patients were asymptomatic and had had no previous contact with COVID-19 infected patients. All semen samples were negative for SARS-CoV-2 RNA. This preliminary assessment suggests that a thorough evaluation (especially in the setting of a multidisciplinary team) and molecular confirmation of the absence of SARS-CoV-2 in seminal fluid from asymptomatic cancer patients may assist in ensuring the safety of sperm cryopreservation.
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